Publication

Abstract : Emergence and spread of MDROs are among the most important global health threats. Surveillance of these pathogens helps to generate local data, to formulate specific infection control procedures. This study was conducted to describe risk factors for acquiring MDRO,their antimicrobial susceptibility pattern, classifying type of ESBL produced using molecular methods in representative E. coli and Klebsiella spp. Methods: Consecutive strains resistant to third generation cephalosporins from HAI/CAI infection were included in the study. A proforma was used for risk factor analysis. Antibiotic susceptibility pattern was determined by Kirby-Bauer disk diffusion method. In 20 strains phenotypic, ESBL detection using cefotaxime, ceftazidime with clavulanic acid by double disk synergy test & E-test (AB. Biodisk, Sweden) was done & genotypic characterisation was done by PCR for TEM, CTX-M, SHV genes.