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Rotational Correlation Time Studies on Nitroxyl Radicals Using 300 MHz ESR Spectrometer in High Viscous Liquid

Publication Type : Journal Article

Publisher : Springer

Source : Applied Magnetic Resonance

Url : https://link.springer.com/article/10.1007/s00723-011-0220-x

Campus : Nagercoil

School : School of Engineering

Year : 2011

Abstract : The mobility studies on 14N-labeled TEMPONE, TEMPO, carbamoyl-PROXYL, carboxy-PROXYL in high viscous liquid were carried out on a 300 MHz electron spin resonance (ESR) spectrometer. The ESR parameters, such as a line width, signal intensity ratio, g-factor, hyperfine coupling constant and correlation time, were determined. The line width broadening increases twofold in high viscous samples of 14N-labeled carbamoyl-PROXYL and carboxy-PROXYL, but this line broadening is negligibly small in the high viscous sample (85% glycerol) of 14N-labeled TEMPO. The correlation time also increases (~30 times) in the high viscous sample (85% glycerol) of 14N-labeled carbamoyl-PROXYL and carboxy-PROXYL, but there is no considerable increase in the high viscous sample of 14N-labeled TEMPO. TEMPONE has the narrowest line width and is also highly sensitive to viscosity. The correlation time increases (~13 times) in the high viscous sample (85% glycerol) of 14N-labeled TEMPONE. Therefore, this study reveals that the 14N-labeled TEMPONE radical is the most suitable spin probe for in vivo studies in high viscous biological fluids.

Cite this Research Publication : Milton Franklin Benial A., Kumara Dhas M., Jawahar A, Rotational Correlation Time Studies on Nitroxyl Radicals Using 300 MHz ESR Spectrometer in High Viscous Liquid, Applied Magnetic Resonance, 40(3), 311-319, 2011.

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