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Publication Type : Journal Article
Publisher : Genes, MDPI
Source : Genes, MDPI, Volume 11, Issue 7, p.763 (2020)
Url : https://pubmed.ncbi.nlm.nih.gov/32650368
Keywords : molecular subtypes, Quantitative proteomics, therapeutic target, urothelial carcinoma
Campus : Amritapuri
School : School of Biotechnology
Department : biotechnology
Year : 2020
Abstract : Bladder carcinoma (BC) incidence and mortality rates are increasing worldwide. The development of novel therapeutic strategies is required to improve clinical management of this cancer. Aberrant protein expression may lead to cancer initiation and progression. Therefore, the identification of these potential protein targets and limiting their expression levels would provide alternative treatment options. In this study, we utilized a liquid-chromatography tandem mass spectrometry-based global proteomics approach to identify differentially expressed proteins in bladder cancer cell lines. A total of 3913 proteins were identified in this study, of which 479 proteins were overexpressed and 141 proteins were downregulated in 4 out of 6 BC cell lines when compared with normal human urothelial cell line (TERT-NHUC). We evaluated the role of UDP-N-acetylhexosamine pyrophosphorylase (UAP1) in bladder cancer pathogenesis. The silencing of UAP1 led to reduction in proliferation, invasion, colony formation and migration capability of bladder cancer cell lines. Thus, our study reveals UAP1 as a promising therapeutic target for bladder cancer.
Cite this Research Publication : V. N. Puttamallesh, Deb, B., Gondkar, K., Jain, A., Dr. Bipin G. Nair, Pandey, A., Chatterjee, A., Gowda, H., and Kumar, P., “Quantitative Proteomics of Urinary Bladder Cancer Cell Lines Identify UAP1 as a Potential Therapeutic Target”, Genes, vol. 11, no. 7, p. 763, 2020.