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Quantitation of mHLA-DR and nCD64 by Flow Cytometry to Study Dysregulated Host Response: The Use of QuantiBRITE™ PE Beads and Its Stability

Publication Type : Journal Article

Publisher : Applied Biochemistry and Biotechnology

Source : Applied Biochemistry and Biotechnology, 2022;1-6. doi:10.1007/s12010-022-03819-6

Url : https://www.citeab.com/publication/33987362-35015219-quantitation-of-mhla-dr-and-ncd64-by-flow-cytometry

Campus : Kochi

School : School of Medicine

Department : C. V. T. S

Year : 2022

Abstract : Quantitation of mHLA-DR and nCD64 is useful in understanding the dysregulated host response. The down regulation of HLA-DR expression on the circulating monocytes (mHLA-DR) is associated with anti-inflammatory response, and an increased expression of CD64 on neutrophil surface (nCD64) is associated with pro-inflammatory response. Quantitation of these antigen expression using beads (QuantiBRITE™ PE) is a precision technique. These beads are reported to be stable for 24 h after reconstitution. We report the results of our investigation examining the stability of QuantiBRITE PE beads over a period of 4-week post-reconstitution. The data suggest that reconstituted QuantiBRITE PE beads, if stored in dark at 2-8 °C, can be effectively used for up to 2 weeks for determining nCD64 and mHLA-DR antibody bound per cell (ABC) values.

Cite this Research Publication : Sanju S, Jain P, Priya VV, Varma PK, Mony U., "Quantitation of mHLA-DR and nCD64 by Flow Cytometry to Study Dysregulated Host Response: The Use of QuantiBRITE™ PE Beads and its Stability," [Published Online ahead of print, 2022 Jan 11]. Applied Biochemistry and Biotechnology, 2022;1-6. doi:10.1007/s12010-022-03819-6

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