Publication Type : Journal Article
Publisher : Applied Biochemistry and Biotechnology
Source : Applied Biochemistry and Biotechnology, 2022;1-6. doi:10.1007/s12010-022-03819-6
Campus : Kochi
School : School of Medicine
Department : C. V. T. S
Year : 2022
Abstract : Quantitation of mHLA-DR and nCD64 is useful in understanding the dysregulated host response. The down regulation of HLA-DR expression on the circulating monocytes (mHLA-DR) is associated with anti-inflammatory response, and an increased expression of CD64 on neutrophil surface (nCD64) is associated with pro-inflammatory response. Quantitation of these antigen expression using beads (QuantiBRITE™ PE) is a precision technique. These beads are reported to be stable for 24 h after reconstitution. We report the results of our investigation examining the stability of QuantiBRITE PE beads over a period of 4-week post-reconstitution. The data suggest that reconstituted QuantiBRITE PE beads, if stored in dark at 2-8 °C, can be effectively used for up to 2 weeks for determining nCD64 and mHLA-DR antibody bound per cell (ABC) values.
Cite this Research Publication : Sanju S, Jain P, Priya VV, Varma PK, Mony U., "Quantitation of mHLA-DR and nCD64 by Flow Cytometry to Study Dysregulated Host Response: The Use of QuantiBRITE™ PE Beads and its Stability," [Published Online ahead of print, 2022 Jan 11]. Applied Biochemistry and Biotechnology, 2022;1-6. doi:10.1007/s12010-022-03819-6