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Publication Type : Journal Article
Publisher : Int J Res Pharm Sci
Source : Int J Res Pharm Sci, Volume 3, p.5–11 (2012)
Keywords : K. P. Rahate, Rajasekaran, A., and Arulkumaran, K. S. G., “Potential of Desmostachya bipinnata Stapf.(Poaceae) root extracts in inhibition of cell proliferation of cervical cancer cell lines”, Int J Res Pharm Sci, vol. 3, pp. 5–11, 2012.
Campus : Kochi
School : School of Pharmacy
Year : 2012
Abstract : Desmostachya bipinnata Stapf (Poaceae), a Sacrificial Grass is an important constituent of ayurvedic preparations of Tripanchamool, Kusablecha, and Kusadya-ghrita. The objective of the study was to evaluate the antioxidant and anticancer activities of Desmostachya bipinnata root extracts. The antioxidant activities of different concentra-tions of 70% methanolic extract of the roots of Desmostachya bipinnata were examined for different reactive oxy-gen species (ROS) scavenging activities including DPPH, nitric oxide, hydrogen peroxide and hydroxyl radical sca-venging activities. The in-vitro cytotoxic study was performed on the human cervical cancer cell lines (HeLa), hu-man laryngeal epithelial carcinoma cells (HEp-2) and NIH 3T3 using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]. The extract has shown effective antioxidant activity in all assay techniques. Further Desmostachya bipinnata was found to be most potent scavenger of hydrogen peroxide with IC 50 value 127.07± 6.44 µg/ml (R 2 0.982) against standard ascorbic acid 122.60± 2.14 µg/ml (R 2 0.984) respectively. The present investigation revealed that the methanolic extract of Desmostachya bipinnata possess significant in vitro anti-cancer activity at 400 μg/ml and showed inhibition in concentration dependant manner of range between 25 – 400 µg/ml on all the cell lines. The results obtained in the present study indicate that the roots of Desmostachya bipinnata are a potential source of natural antioxidants. The study indicated that Desmostachya bipinnata was more active against cervical cell lines.
Cite this Research Publication : K. P. Rahate, Rajasekaran, A., and Arulkumaran, K. S. G., “Potential of Desmostachya bipinnata Stapf.(Poaceae) root extracts in inhibition of cell proliferation of cervical cancer cell lines”, Int J Res Pharm Sci, vol. 3, pp. 5–11, 2012.