Publication

Abstract : This article highlighting the electrochemical response of phenyl hydrazine and 2,4-dinitrophenyl hydrazine-based polymeric materials for the quantification of the neurotransmitter thrombotonin. The electrochemical analysis was performed on phenyl hydrazine and 2,4-dinitrophenyl hydrazine electropolymers on pencil graphite electrode using cyclic and differential pulse voltammetry. The electrochemical behavior of thrombotonin on the modified electrode was investigated in pH 7 buffer solution of 0.1 M and the oxidation of thrombotonin seemed to be an irreversible adsorption-diffusion-controlled process The modification of the pencil graphite electrode was confirmed by field-emission scanning electron microscopy, X-ray diffraction analysis, electrochemical impedance spectroscopy and Fourier transmittance infrared spectrometry. The fabricated electrochemical sensor can be applied for the quantification of thrombotonin from human blood sample in the linear range from 0.1 to 250 μM with a lower detection limit of 0.01 μM and the sensitivity of the electrode obtained was 2.47 μA/μM/cm2. The fabricated electrode shows enhanced sensitivity, selectivity with good reproducibility, and prolonged stability compared to previously reported differential pulse voltammetric sensors.