Programs
- M. Tech. in Automotive Engineering -
- Clinical Fellowship in Laboratory Genetics & Genomics - Fellowship
Publication Type : Journal Article
Publisher : Dalton Trans
Source : Dalton Trans 42(34)12317-12323
Campus : Amritapuri
School : School of Biotechnology
Center : Biotechnology
Department : biotechnology
Verified : Yes
Year : 2013
Abstract : Our recently developed Eu(3+) coordination compound, Eu(pfppd)3(tpy) [where Hpfppd = 4,4,5,5,5-pentafluoro-3-hydroxy-1-(phenanthren-3-yl)pentanedione and tpy = 2,2':6,6''-terpyridine] exhibits significant quantum yield (Φoverall = 41%) and long (5)D0 lifetime (880 μs) values under biologically relevant pH conditions (pH = 7.4) when excited with visible light. Hence, the Eu(3+) luminescent complex was examined for live cell imaging using the rat embryonic heart cell line, H9c2. The ternary Eu(3+) complex permeates into the H9c2 cells and co-localises with the mitochondria, as demonstrated by counterstaining experiments. Furthermore, the designed Eu(3+) bioprobe remains undissociated in the cell medium, showed a good cell permeability and a fast cellular uptake with a specific localization profile. Thus it has the potential to become a time-resolved imaging probe that is excitable in the visible-light range.
Cite this Research Publication : Mitochondria-specific visible-light sensitized europium complex with impressive red emission (2013) Divya V, Sankar V, Raghu KG and Reddy MLP Dalton Trans 42(34)12317-12323 (IF 4.39)