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Knock-down of DMY initiates female pathway in the genetic male medaka, Oryzias latipes

Publication Type : Journal Article

Thematic Areas : Nanosciences and Molecular Medicine

Publisher : Biochemical and biophysical research communications, Academic Press.

Source : Biochemical and biophysical research communications, Academic Press, Volume 351, Number 4, p.815–819 (2006)

Url : http://www.sciencedirect.com/science/article/pii/S0006291X06023230

Keywords : DMY; Germ cell proliferation; Meiosis; Estradiol-17β; Sex reversal; Loss-of-function

Campus : Kochi

School : Center for Nanosciences

Center : Amrita Center for Nanosciences and Molecular Medicine Move, Nanosciences

Department : Nanosciences and Molecular Medicine

Year : 2006

Abstract : DMY is the second vertebrate sex-determining gene identified from the fish, Oryzias latipes. In this study, we used two different ways of sex reversal, DMY knock-down and estradiol-17β (E2) treatment, to determine the possible function of DMY during early gonadal sex differentiation in XY medaka. Our findings revealed that the mitotic and meiotic activities of the germ cells in the 0 day after hatching (dah) DMY knock-down XY larvae were identical to those of the normal XX larvae, suggesting the microenvironment of these XY gonads to be similar to that of the normal XX gonad, where DMY is naturally absent. Conversely, E2 treatment failed to initiate mitosis in the XY gonad, possibly due to an active DMY, even though it could initiate meiosis. Present study is the first to prove that the germ cells in the XY gonad can resume the mitotic activity, if DMY was knocked down.

Cite this Research Publication : Dr. Bindhu Paul, Matsuda, M., Lau, E. -lieng, Suzuki, A., Sakai, F., Kobayashi, T., and Nagahama, Y., “Knock-down of DMY initiates female pathway in the genetic male medaka, Oryzias latipes”, Biochemical and biophysical research communications, vol. 351, pp. 815–819, 2006.

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