Publication

Abstract : Publisher Summary This chapter presents a number of experimental approaches that have proved to be very useful in elucidating the mechanism(s) involved in epidermal growth factor (EGF) -mediated stimulation of cardiac adenylylcyclase. However, there are several additional questions pertaining to the detailed understanding of the mechanism(s) involved in interactions between the {EGF} receptor and Gs interaction. Thus, the in vitro studies demonstrate the juxtamembrane region of the {EGF} receptor that is important for stimulation of Gs by performing mutation(s) of key residue(s) in the juxtamembrane region of the {EGF} receptor, to determine whether the ability of {EGF} to stimulate adenylylcyclase can be obliterated. Similarly, it is important to determine the region(s) of Gsα that interact with the {EGF} receptor. It is also determine that what elements confer specificity to the ability of {EGF} to stimulate adenylylcyclase. The latter goal poses a particularly important question, because {EGF} does not elevate cAMP content in all cells that express the {EGF} receptor, Gs, and adenylylcyclase.