Publication

Abstract : Disposable carbon-based electrochemical sensor was developed for coumarin induced milk toxicity. Coumarin has become a molecule of research interest with regards to its toxicity in food, cosmetic, tobacco and beverages industries. Electroxidation of coumarin were studied using electroanalytical techniques. Anodic peak observed at +0.41 V by differential pulse voltammetry was attributed to the electroxidation of the coumaric acid formed from the lactone ring opening of coumarin in NaOH medium. The possible mechanism for coumarin oxidation in NaOH conditions has been validated using electron transfer involved. Sensor exhibited a linear range of 2–34 µM with a detection limit of 1.3 µM. The sensitivity was found to be 0.4669 µA/µM/cm2 and the selectivity studies revealed response of the sensor is highly selective for coumarin in the presence of other possible interfering molecules. Practical utility of the sensor was depicted in the determination of coumarin from raw milk samples without any pre-treatment.