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Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells

Publication Type : Journal Article

Thematic Areas : Advanced Materials and Green Technologies

Publisher : Chem. Commun

Source : Chemical Communications

Url : https://pubs.rsc.org/en/content/articlelanding/2019/cc/c9cc01483g

Campus : Amritapuri, Coimbatore

School : School of Biotechnology, School of Engineering

Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)

Department : biotechnology, Civil

Year : 2019

Abstract : Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells.

Cite this Research Publication : Bobba KN, Binoy A, Koo S, Nedungadi D, Podder A, Sharma A, Mishra N*, Kim JS*, Bhuniya S* Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells. Chem. Commun., 2019, 55, 6429-6432.

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