Publication

Abstract : This study presents a novel dual electrochemical sensor developed using simple electropolymerization technique to detect and measure homocysteine (HC). The developed electrode modification was confirmed using FTIR, XRD, and SEM-EDX examinations. The poly(N-phenylanthranilic acid) modification of the glassy carbon electrode (GCE) and Screen-printed electrode (SPE) significantly enhanced the response for HC, surpassing the findings of previous studies. The improvement can be credited to the exceptional catalytic efficiency of the electro-polymer in electro-oxidizing HC. The electrochemical investigations were conducted utilizing analytical methods such as cyclic voltammetry and differential pulse voltammetry, with a 0.1 M phosphate buffer solution employed as the supporting medium. The poly-NPAA/GCE and poly-NPAA/SPE sensor has demonstrated exceptional sensing properties such as excellent selectivity, sensitivity, reproducibility and and repeatability. It is highly effective in identifying and quantifying HC. The exhibited linear range of both poly-NPAA/GCE and poly-NPAA/SPE are the widest, ranging from 1.0 to 2300.0 M for poly-NPAA/GCE and from 3.0 to 3000.0 M for poly-NPAA/SPE, making it the most remarkable result ever reported. The detection limit (LOD) of poly-NPAA/GCE was determined to be 0.165 M while that of poly-NPAA/SPE was obtained as 0.618 M. Furthermore, real-sample analysis was performed using biological fluids at the advanced sensor. Therefore, the fabricated poly-NPAA/GCE and poly-NPAA/SPE exhibited exceptional electrocatalytic capabilities for HC sensing, rendering it appropriate for continuous monitoring.