Publication Type : Journal Article
Publisher : Journal of Analysis and Testing
Source : Journal of Analysis and Testing (2024)
Url : https://link.springer.com/article/10.1007/s41664-024-00332-z
Campus : Amritapuri
School : School of Physical Sciences
Department : Chemistry
Year : 2024
Abstract : A simplistic and novel electrochemical sensor for selectively detecting the essential amino acid tryptophan in dietary supplements was fabricated on a pencil graphite electrode modified with a tryptophan-based dipeptide. The dipeptide was synthesized using common synthetic strategy for amino acid couplings. The modified electrode was characterized by scanning electron microscopy, impedance spectroscopy, cyclic voltammetry, and infrared spectroscopy. Differential pulse voltammetry was used to study the electrochemical response of the fabricated electrode to tryptophan in dietary supplements. Under the optimized experimental conditions, a distinct oxidation peak at+0.671 V corresponding to tryptophan was noticed in differential pulse voltammetry, which indicated the detection of tryptophan by the fabricated electrode. The oxidation peak current for tryptophan was found to be linear within a concentration range of 10350 M with a lower detection limit of 1.2 M, and the sensitivity of the fabricated electrode was 6.9 A/M/cm2. Excellent selectivity, permissible reproducibility, and good stability of the sensor make it undoubtedly suitable for determining tryptophan in dietary supplements.
Cite this Research Publication : V. Devika, P. J. Sreelekshmi, B. Vedhanarayanan, Sandhya Sadanandan & R. Rejithamol, Biopolymer Decorated Pencil Graphite Electrode for the In-situ Quantification of L-Tryptophan in Dietary Supplements, Journal of Analysis and Testing (2024)