Publication

Abstract : Used cooking oil is generated as a byproduct during frying process. It cannot be reused for cooking process due to health issues like cancer and other digestive disorders. Alternative strategy is utilization of this waste cooking oil for the production of poly-3-hydroxybutyrate (PHB) or other value added products. In the present investigation waste cooking oil was used as carbon source for PHB production by Bacillus cereus MPTDC. The optimum conditions of PHB production by Bacillus cereus MPTDC were waste cooking oil concentration of 2 % (v/v), incubation time of 96 hrs, ammonium sulphate concentration of 7.5 % and yeast extract concentration of 0.2 %. Under optimized conditions the strain produced 3.777 g/L of PHB. The result of the present study indicates the potential of used cooking oil as carbon source for PHB production by Bacillus cereus MPTDC.