Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine
Publisher : Journal of Molecular Microbiology and Biotechnology
Source : Journal of Molecular Microbiology and Biotechnology, Volume 20, Number 3, p.137-143 (2011)
Keywords : agar diffusion, antimicrobial activity, article, Bacteria (microorganisms), bacterial growth, bacterial strain, bacterium culture, bacterium isolate, biofilm, Biofilms, Cell Line, colon biopsy, Community-Acquired Infections, confocal microscopy, crystal violet, cytotoxicity, growth inhibition, human, human cell, Humans, Lactobacillus, Lactobacillus fermentum, Membrane Proteins, nonhuman, probiotic agent, Probiotics, Pseudomonas aeruginosa, pyocyanine, Staphylococcus aureus, Tumor
Campus : Kochi
School : Center for Nanosciences
Center : Nanosciences
Department : Nanosciences and Molecular Medicine
Year : 2011
Abstract : Surgical wounds and implant-associated Staphylococcus aureus and Pseudomonas aeruginosa infections are often difficult to treat because of limited susceptibility of several of these strains to conventional antibiotics. As a result, there is a constant need for new alternative drugs. The aim of this study was to investigate the antimicrobial properties of Lactobacillus fermentum, a probiotic bacterium, which we have isolated from colonic biopsies. The inhibition of S. aureus and P. aeruginosa growth was evaluated by coincubating with L. fermentum strains. Growth inhibition was tested for several of their clinical isolates using agar well diffusion assays. For biofilm assay S. aureus and P. aeruginosa were grown on the glass slides and in 96-well plates in presence of 2.5 μg/ml culture filtrate of L. fermentum. Biofilms were photographed using confocal microscope or stained with 0.1% crystal violet. Reduction in the cytotoxicity of S. aureus and P. aeruginosa was observed in presence of 2.5 μg/ml L. fermentum-spent media. Using in vitroexperiments, we showed that L. fermentum-secreted compound(s) inhibits the growth, cytotoxicity and biofilm formation of several S. aureus and P. aeruginosa strains. Compound(s) present in the culture supernatant of L. fermentum may have promising applications in treating hospital-acquired infections.
Cite this Research Publication : P. Varma, Nisha, N., Dinesh, K. R., Kumar, A. V., and Biswas, R., “Anti-infective Properties of Lactobacillus Fermentum Against Staphylococcus Aureus and Pseudomonas Aeruginosa”, Journal of Molecular Microbiology and Biotechnology, vol. 20, pp. 137-143, 2011.