Source : Indian Journal Exp Biol 2001
Url : https://nopr.niscpr.res.in/handle/123456789/24039
Campus : Kochi
Year : 2001
Abstract : IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B.Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detection of specific antibody to M. tuberculosis in the sera of patients with PT and controls. The assay gave positive results in all the 12 sputum-smear positive [acid fast bacilli (AFB)] patients with PT and gave negative results in the 50 sera from control groups. The Dot-Iba as described in this study, is simple, rapid and specific for laboratory diagnosis of PT.
Cite this Research Publication : Sumi MG, Annamma M, Reuben S, Radhakrishnan V.V. A Dot immunobinding assay for rapid diagnosis of pulmonary tuberculosis. Indian J Exp Biol 2001, 39:984-988.