Back close

Isolation and Characterization of Host Binding Proteins from Bacillus Clausii and Staphylococcus Aureus Using Mass Spectrometry-a Proteomic Approach

Start Date: Tuesday, Jan 01,2013

End Date: Thursday, May 30,2013

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Dr. Sanjay Pal, Dr. Sudarslal S.
Co-Project Incharge:Arya T V, Nimal S, Rinu S, Thara Menon, Vineeth V
Isolation and Characterization of Host Binding Proteins from Bacillus Clausii and Staphylococcus Aureus Using Mass Spectrometry-a Proteomic Approach

Bacillus clausii, a gram positive, spore forming bacteria is widely used a probiotic in preventing and treating various gastrointestinal disorders, are known to colonize in host systems. But Staphylococcus aureus, though gram positive and show ability to colonize in host systems, is pathogenic in nature. In order to explore the nature of interaction of these bacteria to their hosts, a molecular level study was proposed primarily to isolate and characterize surface layer and secretory proteins. The surface layer proteins were extracted through lithium chloride treatment followed by their isolation and purification by fibronectin and gelatin affinity chromatography. SDS PAGE analysis showed few gelatin binding proteins from Bacillus clausii. Similar observations were made from surface proteins isolated off Bacillus clausii and Staphylococcus aureus through fibronectin affinity chromatography.

Related Projects

Isolation and identification of endophytes from marine algae
Isolation and identification of endophytes from marine algae
Comparison of Anti-microbial Activity of Anti-acne Agents
Comparison of Anti-microbial Activity of Anti-acne Agents
Molecular Structural Studies of Complex Natural Products by LCMS and UV Spectrophotometry
Molecular Structural Studies of Complex Natural Products by LCMS and UV Spectrophotometry
Use of viral agents, microbial fuel cell and effec tive recycling strategy to improve the economics of human waste disposal
Use of viral agents, microbial fuel cell and effec tive recycling strategy to improve the economics of human waste disposal
MFC- Caulobactercrescentus as an Electrogen in A Dual Chambered Microbial Fuel Cell With The Novel Proton Exchange Membrane
MFC- Caulobactercrescentus as an Electrogen in A Dual Chambered Microbial Fuel Cell With The Novel Proton Exchange Membrane
Admissions Apply Now