Back close

Isolation and Characterization of Host Binding Proteins from Bacillus Clausii and Staphylococcus Aureus Using Mass Spectrometry-a Proteomic Approach

Start Date: Tuesday, Jan 01,2013

End Date: Thursday, May 30,2013

School: School of Biotechnology

Thematic Area: Biotech

Project Incharge:Dr. Sanjay Pal, Dr. Sudarslal S.
Co-Project Incharge:Arya T V, Nimal S, Rinu S, Thara Menon, Vineeth V
Isolation and Characterization of Host Binding Proteins from Bacillus Clausii and Staphylococcus Aureus Using Mass Spectrometry-a Proteomic Approach

Bacillus clausii, a gram positive, spore forming bacteria is widely used a probiotic in preventing and treating various gastrointestinal disorders, are known to colonize in host systems. But Staphylococcus aureus, though gram positive and show ability to colonize in host systems, is pathogenic in nature. In order to explore the nature of interaction of these bacteria to their hosts, a molecular level study was proposed primarily to isolate and characterize surface layer and secretory proteins. The surface layer proteins were extracted through lithium chloride treatment followed by their isolation and purification by fibronectin and gelatin affinity chromatography. SDS PAGE analysis showed few gelatin binding proteins from Bacillus clausii. Similar observations were made from surface proteins isolated off Bacillus clausii and Staphylococcus aureus through fibronectin affinity chromatography.

Related Projects

Effect of Volatile Compounds on Pulmonary Pathogens
Effect of Volatile Compounds on Pulmonary Pathogens
Determination of Antibacterial Activity of Different Spices Against E.coli
Determination of Antibacterial Activity of Different Spices Against E.coli
Natural products as modulators of diabetes associated endothelial dysfunctions
Natural products as modulators of diabetes associated endothelial dysfunctions
Benchtop Nanoscale patterning using soft lithography for the printing of DNA molecules
Benchtop Nanoscale patterning using soft lithography for the printing of DNA molecules
Development of Microbial Consortia for Effective Composting
Development of Microbial Consortia for Effective Composting
Admissions Apply Now