Flow of cytometry is a powerful molecular and cellular analytical tool essential for identification in research and clinical applications. Amrita house a state-of-the art Spectral Flow Cytometer from Themro, one of the first in India, capable of sorting nanoscale bioparticles such as exosomes.
This unique workshop offers students, researchers and faculty hands-on exposure to advanced cytometry and molecular techniques. Participants will have the opportunity to engage with Amrita scientists to plan future research. The workshop agenda is available below and all participants will receive a workshop certificate upon completion.
Registration fees for participation is inclusive of course material, lunch, tea
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Category |
Early Registration (Till 31st March 2025) |
On-spot Registration
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PG Students & Scholars |
₹ 1000 |
₹ 1500 |
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Faculty |
₹ 2000 |
₹ 2500 |
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AMRITA RESEARCH CENTRE
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amritarc0710@dlb
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The last date for registration is 31st March 2025.
Send your application along with payment confirmation details to the Workshop Coordinator and research admin :
ashishk.vyas@fbd.amrita.edu | researchadmin@fbd.amrita.edu on or before 31/03/2025
Day 1: Monday April 7, 2025
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Time |
Program |
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8:30 AM-9.00AM |
Registration |
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9:00AM- 9:30 AM |
Inauguration, Overview of workshop and Introduction of participants |
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Theme1:Know Your Flow Cytometer |
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9:30AM- 10:00AM |
Expert Talk on “The Importance of Flow Cytometry in Biomedical Research and Diagnostics” |
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10.00 AM – 11.00 AM |
Understanding the technology of Spectral Flowcytometry and its difference from Conventional Flowcytometry |
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11:00AM – 12:00PM |
Basics of Flowcytometry and cell sorting and its components (principles and instrumentation) |
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12:00PM – 1:30PM |
Practical Demonstration of BigFoot Cell sorter:Components and Operation (including routine checks ofperformance)
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1:30 PM – 2:30 PM |
Lunch |
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Theme 2: Applications of Flowcytometer and cell sorter |
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2:30 PM – 3:00 PM |
Application of Flowcytometer: Cell Cycle; Cell viability; Apoptosis; Immunophenotyping and Applications of Cell sorting |
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3:00 PM-3:30 PM |
Application in Detail: Cell Analysis using PI and Thermo Fisher Vibrant dye cycle |
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3:30 PM-5:30 PM |
Wet Lab: Cell Cycle: Cell Preparation and Staining |
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3:30 PM – 4:30 PM |
Batch 1: Cell Cycle sample run on BigFoot |
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4:30 PM – 5:30 PM |
Batch 2: Cell Cycle sample run on BigFoot |
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5:30 PM – 6:00 PM |
Q & A session |
Day 2: Tuesday April 8, 2025
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Time |
Program |
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9:00AM- 10:00 AM |
Spectral Flowcytometry and Panel Design |
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10:00 AM – 10:30 AM |
Understanding Basic Immunophenotyping: Immune Cell Population and their markers |
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10:30 AM – 11:30 AM |
Application in Detail: Immunophenotyping – Surface and Intracellular: Choice of right fluoro chromes and antibodies, Spectral overlap, Voltage settings, Importance of experiment, data acquisition andcontrols. |
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11:30 AM – 1:30PM |
Wet Lab: Hands-on training in Peripheral Blood Mononuclear Cell Isolation and Discussion |
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1:30 PM – 2:30 PM |
Lunch |
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Batch 1 |
Batch 2 |
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3:30 PM – 4:30 PM |
Wet Lab: Surface Antibody staining of cells |
Acquisition in BigFoot and Analysis |
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Acquisition in BigFoot and Analysis Spectral overlap, |
Wet Lab: Surface Antibody staining of cells |
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4:30 PM – 5:30 PM |
Data Analysis: Cell Cycle and Immunophenotyping |
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5:30 PM – 6:30 PM |
Quiz and Valedictory |
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| Patrons |
Prof. Shantikumar V Nair, |
Prof. Amit Kumar Dinda |
| Workshop Coordinator | Dr. Ashish Kumar Vyas | |
| Organizing Team | Dr. Anubhav Panday, Dr. Pankaj Jha, Dr. Sreeja Roy, Dr. Bhargab Kalita, Dr. Aparup Patra, Dr. Anuj Dilwaria and Mr. Vikas Jha | |
Target Audience
Mode of Selection : First cum first serve basis.
Note: