Back close

Course Detail

Course Name Recombinant DNA Technology
Program BSc. in Microbiology
Semester Five
Credits Two
Year Taught 2019

Syllabus

PART 1

The Basic Principles of Gene Cloning and DNA Analysis   Introduction, History, The advent and importance of gene cloning and the polymerase chain reaction, Vectors for Gene Cloning, Purification of DNA from Living Cells, Manipulation of Purified DNA, Introduction of DNA into Living Cells,

PART 2

Vectors for Cloning

Cloning Vectors for E. coli, λ and other high capacity vectors, Cloning Vectors for Eukaryotes, Genomics & cDNA Libraries

PART 3

Applications and Techniques of Gene Cloning 

Polymerase Chain Reaction & qPCR, Electrophoresis & Blotting Techniques, Site- Directed Mutagenesis, DNA Sequencing, Reporter Gene Assays, DNA-Protein Interaction Assays, Protein-Protein Interaction Assays, DNA Fingerprinting.

Syllabus - Year Taught
2015 2014

Text Books

  1. Gene Cloning and DNA Analysis: An Introduction, 6th Edition, T. A. Brown, Wiley-Blackwel
  2. Principles of Gene Manipulation & Genomics – 7th Edition – Sandy B.Primrose, Richard Twyman– Blackwell

Resources

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now