Preamble
This course will provide a thorough understanding about biology of DNA and how its regulations are brought about, its functional significance and how it is very much involved in the biochemistry and physiology of the cell.
| Course Name | Molecular Biology |
| Course Code | 24MMD502 |
| Program | M. Sc. Molecular Medicine(MLM) |
| Semester | 1 |
| Credits | 3 |
| Campus | Kochi |
This course will provide a thorough understanding about biology of DNA and how its regulations are brought about, its functional significance and how it is very much involved in the biochemistry and physiology of the cell.
DNA: Structure and function, Chromosome and chromatin, Genetic code, wobble hypothesis, RNA and types of RNA (rasiRNA, tasiRNA, nat-siRNA, piRNA), Proteins and their structure
DNA replication and its regulation, Homologous and site-specific recombination, DNA repair
Transcription and its regulation, Translation and its regulation, Gene structure, Repeats and clusters, Gene expression regulations: operon, Epigenetics
Types of mutations, Genetic system of mitochondria
Gene identification, promoter identification, Molecular biology techniques: Isolation and Quantification of DNA/RNA, PCR, Reverse transcriptase PCR, Real Time PCR, DNA Sequence analysis, hybridization (southern, northern and western) and Sanger sequencing
Pre-requisites: Undergraduate level basic DNA biology
Total number of classes: 45
Course Outcome
CO1 Understand the structure and function of DNA, RNA and proteins
CO2 Understand the basics of DNA and RNA replication, transcription, translation
and DNA-repair systems
CO3 Understand how genetic switches work, the basics of gene regulation in
prokaryotes and eukaryotes
CO4 Understand the consequences of different types of mutations and recombinations
CO5 Understand basic and advanced molecular biology concepts and techniques
Program Outcome
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| C | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | 1 | – | – | – | – | – | – | – | – | – | 2 |
| CO 2 | 3 | 1 | – | – | 1 | – | – | – | – | – | – | 1 |
| CO 3 | 3 | 2 | 1 | 2 | 2 | 1 | – | – | – | – | – | 1 |
| CO 4 | 3 | 3 | 1 | 2 | 2 | – | – | – | – | – | – | 2 |
| CO 5 | 3 | 1 | 2 | 1 | 3 | 2 | – | – | – | – | – | 1 |
Program Specific Outcomes. (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular basis of disease
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cellular based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| – | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| C O | ||||||||||
| CO 1 | 3 | 2 | 1 | – | 1 | – | – | – | – | 1 |
| CO 2 | 3 | 2 | 1 | – | 1 | _ | – | – | – | 1 |
| CO 3 | 3 | 2 | 3 | – | 1 | 1 | – | – | – | – |
| CO 4 | 3 | 3 | 3 | 1 | 1 | – | – | – | – | 2 |
| CO 5 | – | 2 | – | 3 | 2 | 2 | – | – | – | 3 |
| Internal Assessment – 50% | ||
| Periodical 1 | Exam | 20% |
| Periodical 2 | Exam | 20% |
| Continuous Assessment | Assignment/Test/Quiz | 10% |
| 50% | ||
| End Semester Examination- 50% | ||
| Theory Exam | 50% | |
| 50% | ||
| Total | 100% |
Lewin’s Essential Genes, Jocelyn E. Krebs, Benjamin Lewin, Elliott S. Goldstein, Stephen T. Kilpatrick,Jones& Bartlett Publishers, 2013 – Science – 847 pages
Molecular Biology of the Gene, Seventh Edition, James D. Watson, Cold Spring Harbor Laboratory; Tania A. Baker, Massachusetts Institute of Technology; Alexander Gann, Cold Spring Harbor Laboratory; Michael Levine, University of California, Berkeley; Richard Losick, Harvard University,2013
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