Course

Practical

Experiment 1: Observation & Identification of Immune Cells,

Experiment 2: Observation and identification of the structure of lymphoid organs,

Experiment 3: Blood grouping,

Experiment 4: Separation of Mononuclear cells using density gradient centrifugation,

Experiment 5: Isolation of Monocytes from the Mononuclear Cells,

Experiment 6: Induction of Monocytes to M1 and M2 Macrophages,

Experiment 7: Evaluation of inflammatory and anti-inflammatory cytokines produced by M1 and M2 macrophages through ELISA.

Experiment 8: Bacterial and fungal culture medium preparation,

Experiment 9: Pure culture technique (eg: streaking and sub culturing),

Experiment 10: Gram staining, Fungal staining, Motility assay

Bacterial growth curve by measuring turbidity and viable count

Experiment 11: Antibiotic sensitivity assay; MIC and MBC determination,

Experiment 12: Biofilm assay, MBIC and MBEC determination,

Experiment 13: Phage titration assay

Preamble

Students will be familiarized with the basics as well as advanced methods in microbiology and immunology that can be used for disease diagnosis. Provide hands-on training for isolation of microbes in pure culture and antibiotic susceptibility assays. Provide the students with practical skills on lymphocyte isolation and analysis.

Pre-requisites: Basic understanding of biology and biochemistry and immunology

Total number of classes:30

Course Outcomes

CO1 Provide the students the knowledge about practical skills on basic microbiology.

CO2 Students will learn about different antimicrobial activity assays.

CO3 Students will develop an understanding about components of immune system

and their function.

CO4 The students will learn about mutagenic assays, biofilm assays and blood grouping.

CO5 The students will learn about different antigen and antibody interaction assays.

Program Outcomes

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

1. Practical Handbook of Microbiology- Lorrence H Green, Emanuel Goldman by CRC Press, 4th Edition, ISBN 9780367567637
2. Practical Immunology-Frank C. Hay, Olwyn M.R. Westwood, Fourth Edition,Blackwell Science.