| Course Name | Molecular Biology Lab |
| Course Code | 22MMD581 |
| Program | M. Sc. Molecular Medicine(MLM) |
| Semester | 1 |
| Credits | 2 |
| Campuses | Faridabad, Kochi |
Isolation of chromosomal DNA from Escherichia coli; Agarose gel electrophoresis,
Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR), Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from Escherichia coli; cDNA synthesis, Reverse Transcriptase PCR, DNA sequencing, Real time PCR.
Pre-requisites: Basic understanding of DNA chemistry and DNA biology
Total number of classes: 30
Course Outcome
CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.
CO2 Can isolate DNA, RNA, plasmids.
CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.
CO4 Can follow general safety routines for laboratory work in molecular biology.
CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.
*Program Outcomes (PO) (As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| C/CO | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| CO1 | 3 | 2 | 2 | 1 | 3 | 1 | 1 | 3 | 1 | |||
| CO2 | 3 | 2 | 3 | 3 | 3 | 1 | 1 | 3 | 1 | |||
| CO3 | 3 | 2 | 3 | 3 | 3 | 1 | 1 | 3 | 1 | |||
| CO4 | 2 | 3 | 3 | 2 | 2 | 1 | 1 | |||||
| CO5 | 3 | 3 | 1 | 2 | 3 | 2 | 1 |
Program Specific Outcomes (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular basis of disease
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cellular based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
| C/CO | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 |
| CO1 | 2 | 1 | 3 | 1 | 2 | 1 | ||||
| CO2 | 3 | 1 | 1 | 1 | ||||||
| CO3 | 3 | 1 | 1 | 1 | ||||||
| CO4 | 3 | |||||||||
| CO5 | 3 |
Evaluation Pattern: 30+70 = 100
| Internal Assessment – 30% | ||
| Records | Evaluation | 30% |
| 30% | ||
| End Semester Examination- 50% | ||
| Practical | Exam | 60% |
| Assessment | Viva | 10% |
| 70% | ||
| Total | 100% | |
Textbooks
Reference
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