Course

‘Medicinal Biochemistry – Practical’ is a course offered in the year semester of Pharm. D. program at School of Pharmacy, Health Sciences campus, Amrita Vishwa Vidyapeetham.

Course Duration: 3 Hrs./Week

List of Experiments:

1. Qualitative analysis of normal constituents of urine.*
2. Qualitative analysis of abnormal constituents of urine.*
3. Quantitative estimation of urine sugar by Benedict’s reagent method.**
4. Quantitative estimation of urine chlorides by Volhard’s method.**
5. Quantitative estimation of urine creatinine by Jaffe’s method.**
6. Quantitative estimation of urine calcium by precipitation method.**
7. Quantitative estimation of serum cholesterol by Libermann Burchard’s method.**
8. Preparation of Folin Wu filtrate from blood.*
9. Quantitative estimation of blood creatinine.**
10. Quantitative estimation of blood sugar Folin-Wu tube method.**
11. Sestimation of SGOT in serum.**
12. Estimation of SGPT in serum.**
13. Estimation of Urea in Serum.**
14. Estimation of Proteins in Serum.**
15. Determination of serum bilirubin**
16. Determination of Glucose by means of Glucoseoxidase.**
17. Enzymatic hydrolysis of Glycogen/Starch by Amylases.**
18. Study of factors affecting Enzyme activity. (pH & Temp.)**
19. Preparation of standard buffer solutions and its pH measurements (any two)*
20. Experiment on lipid profile tests**
21. Determination of sodium,calcium and potassium in serum.**

** indicate major experiments & * indicate minor experiments

Assignments:

Format of the assignment

1. Minimum & Maximum number of pages.
2. It shall be computer draft copy.
3. Reference(s) shall be included at the end.
4. Name and signature of the student.
5. Assignment can be a combined presentation at the end of the academic year.
6. Time allocated for presentation may be 8+2 Min.

Scheme of Practical Examination:

Note : Total sessional marks is 30 (20 for practical sessional plus 10 marks for regularity, promptness, viva-voce and record maintenance).