Introduction to Genetic Engineering – History of Crispr – Crispr in bacteria – Classification of Crispr – General structure of cas9 protein – Mechanism of Crispr cas9 – Applications – Database of Crispr – Case studies.
| Course Name | CRISPR/Cas9 Technology |
| Course Code | 23AID442 |
| Program | B.Tech in Artificial Intelligence and Data Science |
| Credits | 3 |
| Campus | Coimbatore , Amritapuri ,Faridabad , Bangaluru, Amaravati |
Introduction to Genetic Engineering – History of Crispr – Crispr in bacteria – Classification of Crispr – General structure of cas9 protein – Mechanism of Crispr cas9 – Applications – Database of Crispr – Case studies.
Course Objectives
Course Outcomes
After completing this course, students will be able to
|
CO1 |
Analyze and learn the discovery of Crisper with emphasis to molecular mechanisms. |
|
CO2 |
Apply the basic concepts on various application of gene therapy. |
|
CO3 |
Design familiar with experimental design. |
|
CO4 |
create automated pipelines for identifying the associations between multiple genome editions. |
CO-PO Mapping
|
PO/PSO |
PO1 |
PO2 |
PO3 |
PO4 |
PO5 |
PO6 |
PO7 |
PO8 |
PO9 |
PO10 |
PO11 |
PO12 |
PSO1 |
PSO2 |
PSO3 |
|
CO |
|||||||||||||||
|
CO1 |
3 |
3 |
3 |
2 |
3 |
3 |
3 |
3 |
3 |
3 |
3 |
||||
|
CO2 |
3 |
3 |
3 |
2 |
3 |
3 |
3 |
3 |
3 |
3 |
3 |
2 |
|||
|
CO3 |
3 |
3 |
3 |
2 |
3 |
3 |
3 |
3 |
3 |
3 |
3 |
2 |
|||
|
CO4 |
3 |
3 |
3 |
2 |
3 |
3 |
3 |
3 |
3 |
3 |
2 |
3 |
Evaluation Pattern
|
Assessment |
Internal/External |
Weightage (%) |
|
Assignments (Minimum 2) |
Internal |
30 |
|
Quizzes (Minimum 2) |
Internal |
20 |
|
Mid-Term Examination |
Internal |
20 |
|
Term Project/ End Semester Examination |
External |
30 |
Textbooks / References
Maximilian Haeussler, Jean-Paul Concordet, CRISPOR Manual, MIT, 2016. Singh et al: A Mouse Geneticist’s Practical Guide to CRISPR Applications; Genetics, Vol.199, No.1, 2015.
Ran et al, Genome engineering using the CRISPR-Cas9 system, Nature Protocols, 2013.
Fujihara&Ikawaw, CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection, Methods Enzymol. 2014.
DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.