| Course Name | Protein structure-function |
| Course Code | 24MMD341 |
| Program | B. Sc. Honors (with Research) in Molecular Medicine |
| Semester | 6 |
| Credits | 3 |
| Campus | Kochi |
Protein structure and function is a sub-discipline that integrates structural bioinformatics and molecular medicine. This course aims at understanding the biomolecular sequence, structure and function which form the fundamental concepts in structural bioinformatics. This course is divided into different units: Molecular structure of biomolecules; physico-chemical properties of amino acids; Biomolecular structure and function, Pattern identification and recognition.
The amino acid building blocks – classification, structure and physical properties of the standard amino acids; essential and non-essential amino acids; proteinaceous and non-proteinaceous amino acids; optical properties of amino acids; zwitterionic property, isoelectric points and titration curves of amino acids; chemistry of peptide bond; non-ribosomal peptide bond formation; amino acids as precursors of other bioactive compounds; Lambert-Beer Law.
Classification of proteins on the basis of structure, function and composition; conformation of proteins; weak and strong interactions in protein conformation; software involved in judging protein conformation and optimization; basics of protein structure prediction using homology modelling, threading and ab initio modelling.
Primary, secondary (alpha helix, beta sheet, beta turn, collagen helix), tertiary and quaternary structure of biomolecules; Ramachandran Plot; Errat plot; different normal databases and integrated databases in molecular biology; biomolecular structure and function of myoglobin and hemoglobin; molecular physiology of myoglobin and hemoglobin; Bohr effect; Hill’s coefficient.
Concept of lock and key, and induced fit theory; concept of activation energy and binding energy; enzyme kinetics and its physiological significances; enzyme inhibition; types of inhibitors of enzyme with examples.
Symmetry; identification of three dimensional structural pattern; pattern recognition and function of biomolecules.
Unit 5: Post translational modifications in structure function
Course outcome (CO)
CO1: A deeper understanding of amino acid structure, peptide bond chemistry and physic-chemical properties of amino acids
CO2: Understand the conformation of proteins and the basics of protein structure prediction
CO3: Understand the different levels of biomolecular organization
CO4: A deeper understanding of enzyme kinetics and enzyme inhibition
CO5: Significance of bioinformatics in understanding three dimensional structure of proteins
Program outcome (PO)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity
| PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 | |
| CO 1 | 3 | – | – | – | – | – | – | – | – | – | – | 1 |
| CO 2 | 3 | 2 | 2 | 2 | 2 | – | – | – | – | – | – | 1 |
| CO 3 | 3 | – | – | – | – | – | – | – | – | – | – | 1 |
| CO 4 | 3 | 2 | – | 2 | – | 2 | 2 | – | – | – | – | 1 |
| CO 5 | 3 | 2 | 3 | 3 | 3 | – | – | – | – | – | – | 1 |
Program Specific Outcomes.
PSO 1 – Chemical and physical basis of biology
PSO 2 – Computational science in biology and medicine
PSO 3 – Biochemical and physiological complexity in biology and medicine
PSO 4 – Molecular technology in biology and medicine
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in medicine
PSO 7 – Nanoscale entities and its significance in medicine
PSO 8 – Tissue architecture engineering in medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and artificial intelligence in medicine
PSO 11 –Technology in personalizing medicine
PSO 12 – Protein structural complexity in medicine
PSO 13 – Projecting science and medicine to public
| PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 | PSO11 | PSO12 | PSO13 | |
| CO 1 | 3 | – | 1 | – | – | – | – | – | – | – | – | – | – |
| CO 2 | 3 | 3 | 1 | – | – | – | – | – | – | 2 | – | 3 | – |
| CO 3 | 3 | – | 2 | – | – | – | – | – | – | – | – | 3 | – |
| CO 4 | 3 | – | 3 | – | – | – | – | – | 2 | – | – | – | 2 |
| CO 5 | 3 | 3 | – | – | – | – | – | – | – | 3 | – | 3 | – |
|
Internal Assessment – 50% |
||
|
Periodical 1 |
Exam |
20% |
|
Periodical 2 |
Exam |
20% |
|
Continuous Assessment |
Assignment/Test/Quiz |
10% |
|
50% |
||
|
End Semester Examination- 50% |
||
|
Theory Exam |
50% |
|
|
50% |
||
|
Total |
100% |
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