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Course Detail

Course Name Molecular methods
Course Code 24MMD203
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 3
Credits 2
Campus Kochi

Syllabus

Preamble

This course offers a comprehensive exploration of molecular methods crucial in molecular biology. Across five units, students will delve into PCR, cloning, molecular markers, hybridization techniques, and DNA sequencing. From foundational principles to cutting-edge technologies, students will gain practical skills and theoretical understanding to contribute effectively to molecular research. This syllabus serves as a roadmap for students to navigate the complexities of molecular biology with confidence and proficiency.

Unit 1

Polymerase Chain Reaction (PCR): Introduction to PCR principles and components. Detailed exploration of different PCR types: conventional PCR, real-time PCR, quantitative PCR, reverse transcription PCR. Primer design strategies for efficient PCR. Troubleshooting common problems encountered in PCR.

Unit 2

Advanced Cloning Techniques: Principles of DNA cloning. Vector selection and construction strategies. Applications of cloning in research and biotechnology.

Unit 3

Molecular Markers: Introduction to RFLP, RAPD, and AFLP markers. Understanding the characteristics, advantages, and disadvantages of each marker type. Applications of molecular markers in various research fields (e.g., genetic diversity analysis, plant breeding)

Unit 4

Hybridization Techniques: Fundamental principles of nucleic acid hybridization. Southern blotting: detecting specific DNA sequences. Northern blotting: analyzing RNA expression patterns. In-situ hybridization: visualizing DNA or RNA within cells.

Unit 5

DNA Sequencing Techniques: Maxam-Gilbert sequencing method: understanding the chemical principles; Sanger sequencing method: dideoxy termination.

Objectives and Outcomes

Course Outcome

CO1 Understand the principles behind various basic and advanced PCR techniques.

CO2 Understand the fundamentals of molecular cloning.

CO3 Understand how to use the RFLP, RAPD, AFLP PCR based molecular markers for identifying genetic variations.

CO4 Understand the principles behind the different sequencing techniques.

CO5 Understand the technical know-how on versatile hybridization (basic and advanced) techniques.

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

C O

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 3 2 2 2 3 1 2
CO 2 3 2 2 2 3 1 1 2
CO 3 3 2 2 2 3 1 2
CO 4 3 2 2 2 3 1 2
CO 5 3 2 2 2 3 1 2

 

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

C O

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10 PSO11 PSO12 PSO13
CO 1 1 3 3 1 1
CO 2 1 1 3 3 1 2 3 1
CO 3 1 1 3 1 1
CO 4 1 3 3 1 1
CO 5 1 3 2 1 1

Evaluation Pattern

Internal Assessment – 50% 

Periodical 1 

Exam 

20% 

Periodical 2 

Exam 

20% 

Continuous Assessment 

Assignment/Test/Quiz

10% 

50%

End Semester Examination- 50% 

Theory Exam 

50% 

50%

Total

100%

Text Books / References

Molecular Biology Techniques, 4th Edition- A Classroom Laboratory Manual by Sue Carson Heather Miller Melissa Srougi D. Scott Witherow. ISBN: 9780128157756

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