| Course Name | Molecular Diagnostics |
| Course Code | 24MMD343 |
| Program | B. Sc. Honors (with Research) in Molecular Medicine |
| Semester | 6 |
| Credits | 3 |
| Campus | Kochi |
Molecular diagnostics has revolutionized healthcare by providing highly sensitive and specific methods for diagnosing diseases. Building upon the foundational knowledge of molecular biology and methods, this course delves into the practical applications of these techniques in a clinical setting. Students will explore diverse molecular diagnostic assays used to detect pathogens, diagnose genetic disorders, and guide personalized medicine approaches.
Introduction to Advanced Molecular Diagnostics: Overview of Advanced Molecular Diagnostics. Historical Perspective and Recent Advances.
Nucleic Acid Extraction: Methods for isolating high-quality DNA or RNA from blood, saliva, cerebrospinal fluid (CSF), tissues, and other clinical samples.
Advanced Applications and Future Trends: Introduction to next-generation sequencing (NGS) in molecular diagnostics. Microarrays for large-scale gene expression profiling and disease diagnosis.
Molecular diagnostics in oncology: Identification of cancer-causing mutations, targeted therapies). Future directions in molecular diagnostics (e.g., liquid biopsies, digital PCR).
Applications in Infectious Diseases: Molecular diagnosis of bacterial, viral, parasitic, and fungal infections. Antibiotic resistance testing using molecular assays (PCR, Sanger sequencing for specific resistance genes and NGS based resistance detection). Emerging infectious diseases and their molecular diagnostic strategies (e.g., COVID-19 diagnostics).
Applications in Genetic Disorders: Prenatal diagnosis of genetic diseases using chorionic villus sampling (CVS) or amniocentesis coupled with molecular techniques. Carrier testing for inherited diseases using mutation analysis. Pharmacogenomics and personalized medicine based on individual genetic variations.
Course Outcome
CO1 Demonstrate a comprehensive understanding of advanced molecular diagnostic techniques.
CO2 Understand advanced molecular diagnostic methods to diagnose various diseases, including oncological and infectious diseases.
CO3 Evaluate future trends and emerging technologies in molecular diagnostics.
CO4 Design molecular diagnostic strategies for personalized medicine and genetic disorder management.
CO5 Communicate scientific findings and concepts related to advanced molecular diagnostics effectively.
Program Outcome
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
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c C O |
PO1 |
PO2 |
PO3 |
PO4 |
PO5 |
PO6 |
PO7 |
PO8 |
PO9 |
PO10 |
PO11 |
PO12 |
|
CO 1 |
3 |
2 |
2 |
2 |
3 |
1 |
– |
2 |
– |
– |
– |
3 |
|
CO 2 |
3 |
2 |
2 |
2 |
3 |
1 |
1 |
2 |
– |
– |
– |
3 |
|
CO 3 |
3 |
2 |
2 |
2 |
3 |
1 |
– |
2 |
– |
– |
– |
3 |
|
CO 4 |
3 |
2 |
2 |
2 |
3 |
1 |
– |
2 |
– |
– |
– |
3 |
|
CO 5 |
3 |
2 |
2 |
2 |
3 |
1 |
– |
3 |
– |
– |
– |
3 |
Program Specific Outcomes. (PSO)
PSO 1 – Chemical and physical basis of biology
PSO 2 – Computational science in biology and medicine
PSO 3 – Biochemical and physiological complexity in biology and medicine
PSO 4 – Molecular technology in biology and medicine
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in medicine
PSO 7 – Nanoscale entities and its significance in medicine
PSO 8 – Tissue architecture engineering in medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and artificial intelligence in medicine
PSO 11 –Technology in personalizing medicine
PSO 12 – Protein structural complexity in medicine
PSO 13 – Projecting science and medicine to public
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– C O |
PSO1 |
PSO2 |
PSO3 |
PSO4 |
PSO5 |
PSO6 |
PSO7 |
PSO8 |
PSO9 |
PSO10 |
PSO11 |
PSO12 |
PSO13 |
|
CO 1 |
1 |
– |
– |
3 |
– |
– |
– |
– |
– |
3 |
– |
– |
1 |
|
CO 2 |
1 |
3 |
3 |
3 |
1 |
2 |
– |
– |
– |
3 |
3 |
– |
1 |
|
CO 3 |
1 |
2 |
– |
3 |
– |
– |
– |
– |
– |
1 |
3 |
– |
1 |
|
CO 4 |
1 |
– |
– |
3 |
– |
– |
– |
– |
– |
3 |
3 |
– |
1 |
|
CO 5 |
1 |
– |
– |
3 |
– |
– |
– |
– |
– |
2 |
3 |
– |
1 |
|
Internal Assessment – 50% |
||
|
Periodical 1 |
Exam |
20% |
|
Periodical 2 |
Exam |
20% |
|
Continuous Assessment |
Assignment/Test/Quiz |
10% |
|
50% |
||
|
End Semester Examination- 50% |
||
|
Theory Exam |
50% |
|
|
50% |
||
|
Total |
100% |
Molecular Diagnostics: For the Clinical Laboratorian. William B. Coleman (Editor), Gregory J. Tsongalis (Editor) Publisher: Springer-Verlag New York, LLC.
Buckingham and Flaw’s, “Molecular Diagnostics: Fundamentals, Methods and Clinical Applications”, F.A. Davis Company; First edition, 2007.
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