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Course Detail

Course Name Molecular biology lab
Course Code 24MMD185
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 2
Credits 2
Campus Kochi

Syllabus

Experiment
  1. Preparation of various stock solutions required for Molecular Biology Laboratory.
  2. Preparation of culture medium (LB) for E. coli (both solid and liquid) and raise culture of E. coli.
  3. Isolation of chromosomal DNA from bacterial cultures and visualization on Agarose Gel Electrophoresis.
  4. Quantitative estimation of DNA.
  5. Separation of DNA by Agarose gel Electrophoresis
  6. Polymerase Chain Reaction (PCR) technique
  7. Evaluation of the effects of ultraviolet light on bacterial growth.

Objectives and Outcomes

Course Outcome

CO1 Can prepare stock solutions and media components for bacterial culture

CO2 Can explain the principles behind the DNA isolation methods, PCR, agarose gel electrophoresis and UV mutagenesis.

CO3 Can isolate genomic DNA, estimate its quantity and purity. Can perform agarose gel electrophoresis and UV mutagenesis.

CO4 Can follow general safety routines for laboratory work in molecular biology.

CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

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PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

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1

1

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3

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1

CO 2

3

3

3

3

1

1

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3

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1

CO 3

3

2

3

3

3

1

–

–

3

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–

1

CO 4

3

1

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1

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–

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1

CO 5

3

3

2

2

–

2

–

–

–

–

–

1

 

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

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PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

C O

CO 1

1

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1

2

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1

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1

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CO 2

2

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3

2

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1

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1

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CO 3

3

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3

3

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1

–

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–

–

1

–

1

CO 4

–

–

1

1

–

–

–

–

–

–

–

–

1

CO 5

–

–

1

1

–

–

–

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–

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1

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 
   

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%
   

70%
 

Total

100%

Text Books / References

(1) Cell and Molecular Biology: Concepts and Experiments, 7th edition (2013), Gerald Karp; Wiley Publishers ISBN-13: 978-1118206737.

(2) Molecular Cloning: A Laboratory Manual, 4th edition (2012), Michael R. Green and Joseph Sambrook; Cold Spring Harbor Laboratory Press, ISBN-13: 978-1936113422

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