Preamble
Molecular Biology encompasses the basic study and understanding of the execution of central dogma.
| Course Name | Molecular biology |
| Course Code | 24MMD112 |
| Program | B. Sc. Honors (with Research) in Molecular Medicine |
| Semester | 2 |
| Credits | 2 |
| Campus | Kochi |
Molecular Biology encompasses the basic study and understanding of the execution of central dogma.
Historical and conceptual background: Discovery of DNA as genetic material, Griffith’s experiment, Hershy and Chase experiment, Chargaff’s rule and DNA double helix.
Genome organization: Structure of DNA, Types of DNA, Organization of Chromatin. Concept of Gene, Introns and Exons genome, chromosome. Structure of RNA and Protein. Nature and Properties of Genetic Code.
The replication of DNA: Chemistry of DNA synthesis, Mechanism of Replication: Initiation, Elongation, synthesis of Leading and lagging strands, Termination. Enzymes and proteins involved in DNA replication.
The mutability and Repair of DNA: Replication Errors, DNA Damage and their repair: mismatch repair, SOS response, Excision Repair, Photoreactivation. Types of mutations.
Information Transfer –I: Mechanism of Transcription: Basic transcription apparatus, Initiation, elongation and termination of transcription, Transcription of mRNA, tRNA and rRNA, types of RNA polymerases, transcription factors, Inhibitors of transcription- rifampicin and α-amanitin. Post-Transcriptional Modifications: Split Genes, Concept of introns and exons, RNA splicing, Spliceosomes, Gene regulation.
Mechanism of Translation: Features of genetic code and exceptions in some systems, Ribosome structure- rRNA and proteins, Charging of tRNA, aminoacyl tRNA synthetases, Proteins involved in initiation, elongation and termination of polypeptides, Fidelity of translation, Inhibitors of protein synthesis – tetracyclins, aminoglycosides, chloramphenicol and aminoglycosides.
Course Outcome
CO1 – Understand the structure and function of DNA, RNA, proteins and genome organization in prokaryotes and eukaryotes. Understand the concept of Gene and the gene architecture.
CO2 – Understand the role of different enzymes and the molecular events in the DNA replication.
CO3 – Understand the DNA-repair systems and consequences of different types of mutations.
CO4 – Understand the key molecular events of transcription, post-transcriptional processing of eukaryotic transcripts. Understand the basics of gene regulation.
CO5 – Understand the molecular events of translation and post translational modification.
Program Outcome
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| c | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | 1 | – | – | – | – | – | – | – | – | – | 2 |
| CO 2 | 3 | 1 | – | – | – | – | – | – | – | – | – | 2 |
| CO 3 | 3 | 2 | – | – | – | – | – | – | – | – | – | 2 |
| CO 4 | 3 | 2 | – | – | – | – | – | – | – | – | – | 2 |
| CO 5 | 3 | 2 | – | – | – | – | – | – | – | – | – | 2 |
Program Specific Outcomes. (PSO)
PSO 1 – Chemical and physical basis of biology
PSO 2 – Computational science in biology and medicine
PSO 3 – Biochemical and physiological complexity in biology and medicine
PSO 4 – Molecular technology in biology and medicine
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in medicine
PSO 7 – Nanoscale entities and its significance in medicine
PSO 8 – Tissue architecture engineering in medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and artificial intelligence in medicine
PSO 11 –Technology in personalizing medicine
PSO 12 – Protein structural complexity in medicine
PSO 13 – Projecting science and medicine to public
| – | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 | PSO11 | PSO12 | PSO13 |
| C O | |||||||||||||
| CO 1 | 3 | 2 | 3 | 1 | – | – | – | – | – | – | – | 2 | 1 |
| CO 2 | 3 | 1 | 3 | 1 | 1 | 1 | – | – | – | – | – | – | 1 |
| CO 3 | 3 | 2 | 3 | 1 | 1 | 1 | – | – | – | 1 | – | 2 | 1 |
| CO 4 | 3 | 1 | 3 | 1 | 1 | – | – | – | – | – | – | – | 1 |
| CO 5 | 3 | 1 | 3 | 1 | 1 | – | – | – | – | – | – | – | 1 |
|
Internal Assessment – 50% |
||
|
Periodical 1 |
Exam |
20% |
|
Periodical 2 |
Exam |
20% |
|
Continuous Assessment |
Assignment/Test/Quiz |
10% |
|
50% |
||
|
End Semester Examination- 50% |
||
|
Theory Exam |
50% |
|
|
50% |
||
|
Total |
100% |
(1) Friefelder, D. Molecular Biology.2nd Edition.Narosa Book Distributors Pvt. Ltd. 2008.
(2) Alberts, B., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P. Molecular Biology of the Cell. 5th Edition. Garland Science. 2007.
(3) Molecular Biology of the Gene, 6th edition (2007), Watson, J. D., Baker T. A., Bell, S. P., Gann, A., Levine, M., and Losick, R; Benjamin Cummings Publishers, ISBN-13: 978- 0805395921.
(4) The Cell: A Molecular Approach, 6th edition (2013), Cooper and Hausman; Sinauer Associates, Inc. ISBN-13: 978-1605351551.
DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.