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Course Detail

Course Name Microbiology lab
Course Code 24MMD184
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 2
Credits 2
Campus Kochi

Syllabus

Experiment
  1. Isolation of bacteria
  2. Simple staining
  3. Gram Staining
  4. Acid fast Staining
  5. Spore Staining
  6. Negative Staining
  7. Preparation of media & sterilization methods
  8. Demonstration of microflora in air by plate exposure
  9. Enumeration of microorganisms – viable count
  10. Antimicrobial activity assays – Agar diffusion
  11. Antimicrobial activity assays – Broth dilution
  12. Alamar blue method for viability determination
  13. Bacteriophage isolation

Objectives and Outcomes

Course Outcome

CO1 – Apply the knowledge to isolate and identify microorganisms.

CO2 – The students will learn to perform antibiotic susceptibility assays.

CO3 – The students will learn to perform mutagenesis experiment for the identification of potential carcinogenic/mutagenic compounds.

CO4 – The students will learn to perform microbial viability assays.

CO5 – They will learn different bacterial staining procedures.

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
C O
CO 1 3 2 3
CO 2 3 2 3
CO 3 3 1 1 1 1 2 3
CO 4 3 1 1 1 2 3
CO 5 3 1 2 3

 

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10 PSO11 PSO12 PSO13
C O
CO 1 1 3
CO 2 2 3 3
CO 3 2 3
CO 4 2 3
CO 5 2 3

Evaluation Pattern

Internal Assessment – 30%
Records Evaluation 30%
30%
End Semester Examination- 50%
Practical Exam 60%
Assessment Viva 10%
70%
Total 100%

Text Books / References

  1. Bailey & Scott’s Diagnostic Microbiology, 14th Edition.

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