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Course Detail

Course Name Cell biology lab
Course Code 24MMD182
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 1
Credits 2
Campus Kochi

Syllabus

Experiment

This lab is aimed to demonstrate use of microscopy in the study of cells, the morphology and internal organization of various types of cells, permeability of plasma membrane and different phases in mitosis and meiosis.

( 1 ) Drawing of ultrastructure of cell and different organelles (from photographs provided).

( 2 ) Familiarization with the student’s light microscope and stereobinocular microscope.

( 3 ) Application of centrifuge – separation of sperm from other testicular cells by low speed centrifugation.

( 4 ) Diversity of eukaryotic cells – methylene blue staining of buccal epithelium, sperm, neurons, striated muscle cells; Leishman staining of mammalian blood cells.

( 5 ) Permeability of plasma membrane – effect of isotonic, hypotonic and hypertonic solutions on mammalian RBC.

( 6 ) Staining of nucleolus (RNA) and chromatin (DNA) with methyl green-pyronin Y.

( 7 ) Staining of mitochondria with Janus green in buccal epithelium.

( 8 ) Mitosis using somatic cells.

( 9 ) Meiosis using germ cells.

Objectives and Outcomes

Course Outcomes

CO1 – To learn about light microscope in deciphering the basic structure of cells

CO2 – To learn how to use a centrifuge to separate different types of cells

CO3 – To understand the ultrastructure of cells and observe the differences between various types of eukaryotic cells through different staining techniques

CO4 – To observe the effect of isotonic, hypotonic and hypertonic solutions on membrane permeability

CO5 – To differentiate mitotic and meiotic cells through observation under microscope

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
C O
CO 1 3 1 2 2 2 3 2 3
CO 2 3 1 2 2 2 3 2 3
CO 3 3 1 2 2 2 3 2 3
CO 4 3 1 2 2 2 3 2 3
CO 5 3 1 2 2 2 3 2 3

3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10 PSO11 PSO12 PSO13
C O
CO 1 3 1 3 2 3 1 2 1 2 3
CO 2 3 1 3 2 3 1 2 1 2 3
CO 3 3 1 3 2 3 1 2 3 2 3
CO 4 3 1 3 2 3 1 2 1 2 3
CO 5 3 1 3 2 3 1 2 1 2 3

Evaluation Pattern

Internal Assessment – 30%
Records Evaluation 30%
30%
End Semester Examination- 50%
Practical Exam 60%
Assessment Viva 10%
70%
Total 100%

Text Books / References

( 1 ) Karp: Cell and Molecular Biology ( 2008, John Wiley ).

( 2 ) Lodish et al: Molecular Cell Biology ( 2008, Freeman ).

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