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Course Detail

Course Name Biotechniques lab
Course Code 24MMD283
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 4
Credits 2
Campus Kochi

Syllabus

Experiment
  1. Protein purification
  2. Chromatography
  3. SDS_PAGE
  4. Measurement of Molecular weight
  5. Protein estimation
  6. Western blotting
  7. Chemiluminescence reaction
  8. Image capturing following chemiluminescence reaction

Objectives and Outcomes

Course Outcome

CO1 Understanding the principles applied to protein purification and its significance.

CO2 Student will understand different strategies applied towards proteins purification.

CO3 Different techniques currently used in characterization of proteins.

CO4 Significance of different technologies used in protein identification, characterization and purification.

CO5 Understand the different instruments used in protein identification and purifications.

Program outcomes

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

2

1

1

1

1

–

–

–

–

–

3

CO 2

3

2

3

2

1

1

–

–

–

–

–

3

CO 3

3

2

3

3

3

2

–

–

–

–

–

3

CO 4

3

2

3

3

3

1

–

–

1

–

–

3

CO 5

3

3

3

3

3

1

–

–

–

–

–

3

 

3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

Program Specific Outcomes.

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

C O

CO 1

1

–

–

3

–

–

–

–

–

–

2

3

3

CO 2

–

–

–

3

–

–

–

–

–

–

1

3

3

CO 3

–

–

–

3

–

–

–

–

–

1

2

3

3

CO 4

–

–

–

3

–

–

–

–

–

–

1

3

3

CO 5

–

–

–

3

–

–

–

–

–

–

1

3

3

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%

70%

Total

100%

Text Books / References

  1. Introductory Practical biochemistry, S. K. Sawhney & Randhir Singh (eds) Narosa Publishing House, New Delhi, ISBN 81-7319-302-9, p 1- 15
  2. Hawk’s Physiological Chemistry, Bernard L. Oser (ed) TATA McGRAW Hill Publishing Company LTD, New Delhi, p 10- 15.
  3. Protein Purification Principles and Practice by Robert K. Scopes. Publisher, Springer New York, NY. 3rd Edition

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