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Course Detail

Course Name Biotechniques
Course Code 24MMD215
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 4
Credits 2
Campus Kochi

Syllabus

Preamble

Biotechniques are the key methods responsible for the advancement towards deciphering science and development of methods for diagnosis, prognosis and therapies in clinics.

Unit 1

Purification methods and its requirements in science and medical applications, Affinity chromatography, Metal ion binding Immobilized metal ion affinity chromatography, Charge Ion exchange chromatography, Size Gel filtration, Hydrophobicity, Hydrophobic interaction chromatography, Reversed phase chromatography,

Unit 2

Isoelectric point, Chromato focusing, chromatography media, buffers for chromatography, Poly-acrylamide gel electrophoresis, Blotting techniques.

Unit 3

Principles of fast performing liquid chromatography (FPLC) and high performing liquid chromatography (HPLC), use of FPLC and HPLC and its applications in science and industry. Mass spectrometry, principles of mass spectrometry, its applications and advantages. Use of mass spectrometry in clinics

Objectives and Outcomes

Course Outcome

CO1 Develop an insight into the protein composition and how charges are distributed on it what how three-dimensional structures proteins plays a role in protein purification.

CO2 Student will learn protein characteristics such as charge, affinity, size and specific interactions and its use in purification.

CO3 Different techniques currently used in characterization of proteins and to identify its characteristics using different techniques.

CO4 Significance of different technologies used in protein identification, characterization and purification.

CO5 Instrumentation used in protein identification and purifications based on protein characteristics.

Programme Outcomes (PO) (As given by NBA and ABET)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

2

2

1

2

1

–

–

–

–

–

2

CO 2

3

1

2

1

2

–

–

–

–

–

–

2

CO 3

3

2

2

2

3

–

–

–

–

–

–

2

CO 4

3

3

3

3

3

–

–

–

–

–

–

3

CO 5

2

2

3

3

3

–

–

–

–

–

–

3

 

3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

C O

CO 1

3

–

2

–

–

–

–

–

–

–

–

2

2

CO 2

3

–

1

–

–

–

–

–

–

–

–

2

2

CO 3

1

–

1

3

–

–

–

–

–

–

–

2

2

CO 4

1

–

1

3

–

–

–

–

–

–

–

2

2

CO 5

–

–

–

3

–

–

–

–

–

–

–

2

2

Evaluation Pattern

Internal Assessment – 50% 

Periodical 1 

Exam 

20% 

Periodical 2 

Exam 

20% 

Continuous Assessment 

Assignment/Test/Quiz

10% 

50%

End Semester Examination- 50% 

Theory Exam 

50% 

50%

Total

100%

Text Books / References

  • Paul Cutler; Protein Purification Protocols, Humana Press.
  • Protein Purification Principles and Practice, Springer New York, NY. 3rd Edition

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