| Course Name | Biochemistry lab |
| Course Code | 24MMD181 |
| Program | B. Sc. Honors (with Research) in Molecular Medicine |
| Semester | 1 |
| Credits | 2 |
| Campus | Kochi |
( 1 ) Preparation of buffers;
( 2 ) Determination of pKa value;
( 3 ) Estimation of proteins by Biuret method;
( 4 ) Estimation of proteins by Lowry’s method;
( 5 ) Separation of sugars by Thin Layer chromatography,
( 6 ) Effect of pH and temperature on the activity of an enzyme,
( 7 ) Progress curve of an enzyme.
Course Outcomes ( CO )
CO1 – Develop basic understanding of chemical interactions helping in stabilizing the biological components.
CO2 – Understanding the principles of protein analysis.
CO3 – Exposing the students to how principles of biochemistry are translated to different techniques of protein analysis.
CO4 – Understanding how the environment around enzymes influencing its activity and how and how it can be studied.
CO5 – Enable students to realize the significance proteins as enzymes and its evaluation.
Program Outcomes ( PO )
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
| c | PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
| C O | ||||||||||||
| CO 1 | 3 | 1 | 3 | 1 | – | – | – | – | 1 | – | – | 2 |
| CO 2 | 3 | 1 | 3 | 1 | – | 1 | – | – | – | – | – | 2 |
| CO 3 | 3 | 2 | 3 | 1 | 2 | 1 | – | – | 1 | – | – | 2 |
| CO 4 | 3 | 1 | 1 | 1 | – | 1 | – | – | 1 | – | – | 2 |
| CO 5 | 3 | 1 | – | 2 | – | 1 | – | – | – | – | – | 2 |
3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity
Program Specific Outcomes ( PSO )
PSO 1 – Chemical and physical basis of biology
PSO 2 – Computational science in biology and medicine
PSO 3 – Biochemical and physiological complexity in biology and medicine
PSO 4 – Molecular technology in biology and medicine
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in medicine
PSO 7 – Nanoscale entities and its significance in medicine
PSO 8 – Tissue architecture engineering in medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and artificial intelligence in medicine
PSO 11 – Technology in personalizing medicine
PSO 12 – Protein structural complexity in medicine
PSO 13 – Projecting science and medicine to public
| – | PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 | PSO11 | PSO12 | PSO13 |
| C O | |||||||||||||
| CO 1 | 3 | – | – | 1 | – | – | – | – | – | – | – | – | 2 |
| CO 2 | 2 | – | 1 | 1 | – | – | – | – | – | – | 1 | – | 2 |
| CO 3 | 1 | – | 1 | 3 | – | – | – | – | – | 2 | 2 | – | 2 |
| CO 4 | 2 | – | 1 | 1 | – | – | – | – | – | – | – | 1 | 1 |
| CO 5 | 1 | – | 2 | 1 | – | – | – | – | – | – | – | 1 | 2 |
|
Internal Assessment – 30% |
||
|
Records |
Evaluation |
30% |
|
30% |
||
|
End Semester Examination- 50% |
||
|
Practical |
Exam |
60% |
|
Assessment |
Viva |
10% |
|
70% |
||
|
Total |
100% |
( 1 ) Biochemistry in the Lab A Manual for Undergraduates by Benjamin F. Lasseter.
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