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Course Detail

Course Name Bio Physics lab
Course Code 24MMD183
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 2
Credits 2
Campus Kochi

Syllabus

Experiment
  1. Acquire the absorption spectrum of the given sample using spectrophotometer.
  2. Effect of temperature, pH and solvents on UV absorption spectra of proteins using Spectrophotometry.
  3. Determination of the absorption maxima of pNitro phenol, DNA and protein (BSA/Egg Albumin) from absorption spectrum and calculate molar absorbance.
  4. Determination of the concentrations of given test samples using Beer Lambert Law.
  5. Determination of the concentrations of given test samples using calibration curve.
  6. Determination of the specific rotation of sugar solution by using Laurent’s Half Shade Polarimeter.
  7. Determination of the concentrations of given test samples using Laurent’s Half Shade Polarimeter.
  8. Determination of the refractive index of the given samples using Abbe Refractometer.
  9. Determination of the concentrations of given test samples using calibration curve using Abbe Refractometer.

Objectives and Outcomes

Course Outcome

CO1 Standard curve for concentration analysis

CO2 UV -Vis Absorption Spectroscopy for concentration analysis

CO3 Effect of pH and temperature on UV-Vis Absorption Spectrum of proteins

CO4 Refractive index of solutions for concentration analysis

CO5 Optical rotation of solutions for concentration analysis

Program Outcomes (PO) (As given by NBA and ABET)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bio Scientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

1

2

2

1

2

2

–

–

2

–

–

1

CO 2

2

1

2

–

3

1

–

–

2

–

–

1

CO 3

2

1

1

–

3

1

–

–

2

–

–

–

CO 4

1

2

2

  

2

      

2

  

–

1

CO 5

1

2

2

1

2

–

–

–

2

  

–

1

 

3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

Program Specific Outcomes.(PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 -Computational science in biology and medicine

PSO 3 -Biochemical and physiological complexity in biology and medicine

PSO 4 -Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 -Microorganisms in medicine

PSO 7 -Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

C O

CO 1

–

1

–

–

–

–

–

–

1

1

1

–

–

CO 2

1

1

–

–

–

–

–

–

1

1

1

1

–

CO 3

2

1

–

–

–

–

–

–

1

1

1

1

1

CO 4

2

1

–

–

–

–

–

–

1

1

1

–

1

CO 5

2

1

–

–

–

–

–

–

1

1

1

–

1

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%

70%

Total

100%

Text Books / References

Biophysical Chemistry (2013), Schimmel, C.R.C., Macmillan Higher Education, ISBN : 0716738619, 9780716738619.

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