Publication Type : Journal Article
Thematic Areas : Advanced Materials and Green Technologies
Publisher : New Journal of Chemistry, Royal Society of Chemistry .
Source : New Journal of Chemistry, Royal Society of Chemistry, Volume 42, Issue 3, p.1590–1594 (2018)
Url : https://pubs.rsc.org/en/content/articlelanding/2018/nj/c7nj04119e#!divAbstract
Campus : Coimbatore
School : Center for Industrial Research and Innovation, School of Engineering
Center : Center for Excellence in Advanced Materials and Green Technologies, Center for Industrial Research and Innovation (ACIRI)
Department : Civil, Industrial Research & Innovation
Year : 2018
Abstract : We developed a naphthalimide-based, lysosome-targeting, and self-immolative fluorescent probe for H2S detection. Probe LS-1 comprises a 2-formylbenzoate derivative of the 1,8-naphthalimide fluorophore. H2S forms a thiohemiacetal intermediate by reacting with the formyl group of the 2-formylbenzoate derivative, which subsequently undergoes intramolecular cyclization with the ester moiety to generate a free naphthalimide fluorophore (FL-1). The UV-vis absorbance (λabs) value of probe LS-1 at 450 nm increased in the presence of H2S. Similarly, with increasing H2S concentrations, the emission band (λem) centered at 560 nm increased gradually. The probe was found to be highly sensitive and chemoselective towards H2S compared with other biological analytes. Probe LS-1 was nontoxic and very stable across the physiological pH range. The probe LS-1 enables the detection of intracellular endogenous H2S formation in HT29 cells in lysosomes.
Cite this Research Publication : N. Velusamy, Thirumalaivasan, N., Bobba, K. Naidu, Wu, S. - P., and Bhuniya, S., “A hydrogen sulfide triggered self-immolative fluorescent probe for lysosome labeling in live cells”, New Journal of Chemistry, vol. 42, no. 3, pp. 1590–1594, 2018.