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Course Detail

Course Name Analytical technique and instrumental methods in soil and plant analysis
Course Code 25SAC510
Program M. Sc. (Agriculture) in Agronomy
Credits 2
Campus Coimbatore

Syllabus

Unit I
Theory

Preparation of solutions for standard curves, indicators and standard solutions for acid-base, oxidation reduction and complexometric titration; soil, water and plant sampling techniques, their processing and handling.

Unit II

Determination of nutrient potentials and potential buffering capacities of soils for phosphorus and potassium; estimation of phosphorus, ammonium and potassium fixation capacities of soils.

Unit III

Principles of visible, ultra violet and infrared spectrophotometery, atomic absorption, flame-photometry, inductively coupled plasma spectrometry; chromatographic techniques, mass spectrometry and X-ray defractrometery; identification of minerals by X-ray by different methods, CHNS analyzer.

Unit IV

Electrochemical titration of clays; estimation of exchangeable cations (Na, Ca, Mg, K); estimation of root cation exchange capacity.

Unit V

Wet digestion/fusion/extraction of soil with aquaregia with soil for elemental analysis; triacid/di-acid digestion of plant samples; determination of available and total nutrients (N, P, K, S, Ca, Mg, Zn, Cu, Fe, Mn, B, Mo) in soils; determination of total nutrients (N, P, K, S, Ca, Mg, Zn, Cu, Fe, Mn, B, Mo) in plants

Unit VI

Drawing normalized exchange isotherms; measurement of redox potential.

Aim of the course

To familiarize the students with commonly used instruments – their working, preparations of common analytical reagents for qualitative and quantitative analysis of both soil as well as plant samples.

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