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Course Detail

Course Name Cell culture lab
Course Code 24MMD284
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 4
Credits 2
Campus Kochi

Syllabus

Experiment

(1) Fundamentals of cell culture.

(2) Facilities and Applications.

(3) Media preparation for Animal cells culture.

(4) Types of cell culture: Primary and secondary cell culture, cell lines, stem cell cultures,

(5) Tests: cell viability and cytotoxicity,

(6) Cryopreservation.

Objectives and Outcomes

Course outcome (CO)

CO1: Understand the fundamentals of cell culture and its applications

CO2: Familiarize with cell culture facilities and equipments

CO3: Understand the composition of cell culture media and their significance

CO4: Basic understanding of different types of cell culture

CO5: Significance of cell viability and cell cytotoxicity assays in biological studies

Program outcome (PO)

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

3 = High Affinity, 2 = Medium Affinity, 1 = Low Affinity, – = No Affinity

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

CO 1

3

–

–

–

–

–

–

3

–

–

–

1

CO 2

3

–

–

–

1

–

–

–

–

–

–

1

CO 3

3

–

–

–

–

–

–

–

–

–

–

1

CO 4

3

–

–

–

–

–

–

–

–

–

–

1

CO 5

3

2

–

2

–

2

–

–

1

–

–

1

 

Program Specific Outcome (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 – Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

CO 1

3

–

3

–

–

–

–

–

–

–

–

–

–

CO 2

–

–

–

–

–

–

–

–

–

–

–

–

–

CO 3

–

–

–

–

–

–

–

–

–

–

–

–

–

CO 4

3

–

2

–

2

–

–

–

–

–

–

–

–

CO 5

3

–

2

–

3

–

–

–

3

–

–

–

2

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%

70%

Total

100%

Text Books / References

(1) Basic Cell Culture Protocols (3rd edition) by Cheryl D. Helgason, Cindy L. Miller.

(2) Masters, J.R.W. Animal Cell Culture: A Practical Approach. 3rd Edition.Oxford University Press. 2000.

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