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Course Detail

Course Name Gene Editing Technology
Course Code 24MMD411
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester VII
Credits 2
Campus Kochi

Syllabus

Preamble

Genome editing provides the ability to manipulate gene expression in a variety of cell types and animal models. This course will provide an overview of the current major genome engineering platforms and cover CRISPR genome editing technology in depth.

Unit 1

Introduction to Genome Editing

Unit 2

DNA Repair Mechanisms in Genome Editing

Unit 3

Genome Editing tools: Restriction enzymes, Zinc finger nucleases (ZFNs), TALENs, CRISPR-Cas9

Unit 4

ZFNs and TALENs: Design and assembly, Functional testing of assembled designer tools, Applications in genome editing, Limitations

Unit 5

Origins of CRISPR, Introduction to CRISPR Technology, sgRNA Design Considerations, sgRNA and Cas9 Delivery into Target Cells, Validation of CRISPR Gene Editing, CRISPR Knockout Case Study, Clinical Applications, The Future of CRISPR and Ethical Considerations (CRIPSR in the Clinic, CRISPR Babies, Case-Studies)

Unit 6

Ethics: Moral considerations for applications of genomic editing, policies and guidelines for gene modifications. Biosafety Considerations. Regulatory Issues and Future of Genome -Editing Technology

Objectives and Outcomes

Course Outcome

CO1 Understand the role of DNA repair mechanisms in genome editing. Understand the principles and mechanisms of different genome editing tools (ZFNs, TALENs & CRISPR-Cas9).

CO2 Learn how to design optimal target-specific ZFNs, TALENS and gRNA molecules to edit genes of interests.

CO3 Learn how to deliver the genome editing components using various transfection methods.

CO4 Understand how to measure the editing efficiency with a mismatch detection assay.

CO5 Understand the ethics and biosafety of gene editing technologies.

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

2

2

2

3

1

–

–

–

–

–

2

CO 2

3

2

2

2

3

1

1

–

–

–

–

2

CO 3

3

2

2

2

3

1

–

–

–

–

–

2

CO 4

3

2

2

2

3

1

–

–

–

–

–

2

CO 5

3

2

2

2

3

1

–

3

–

–

–

2

 

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

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PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

PSO11

PSO12

PSO13

C O

CO 1

3

1

2

3

1

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–

–

3

3

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1

CO 2

1

1

3

3

1

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–

3

1

–

1

CO 3

1

1

3

3

2

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–

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–

–

1

–

1

CO 4

1

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3

–

–

–

–

–

1

1

–

1

CO 5

–

–

1

–

–

–

–

–

–

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1

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1

Evaluation Pattern

Internal Assessment – 50%
Periodical 1 Exam 20%
Periodical 2 Exam 20%
Continuous Assessment Assignment/Test/Quiz 10%
  50%
End Semester Examination- 50%
Theory Exam 50%  
  50%
Total 100%

Text Books / References

  1. Targeted Genome Editing Using Site-Specific Nucleases, ZFNs, TALENs, and the CRISPR/Cas9 system by Takashi Yamamoto; ISBN: ISBN 978-4-431-55226-0; Publisher: Springer Singapore.
  2. Precision Medicine, CRISPR, and Genome Engineering by Stephen H. Tsang; ISBN 978-3-319-63903-1; Publisher: Springer Singapore

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