Registration fees for participation is inclusive of course material, lunch, tea
|
Category |
Early Registration (Till 15th March 2025) |
Late Registration (After 15th March 2025) |
|
PG Students & Scholars |
₹ 3500 |
₹ 4000 |
|
Faculty |
₹ 4500 |
₹ 5000 |
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AMRITA RESEARCH CENTRE
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amritarc0710@dlb
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The last date for registration is 31st March 2025.
Send your application along with payment confirmation details to the Workshop Coordinator and research admin :
ashishk.vyas@fbd.amrita.edu | researchadmin@fbd.amrita.edu on or before 31/03/2025
Day 1: Monday April 7, 2025
|
Time |
Program |
|
8:30 AM-9.00AM |
Registration |
|
9:00AM- 9:30 AM |
Inauguration,Overviewofworkshopand Introductionofparticipants |
|
Theme1:KnowYourFlowCytometer |
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|
9:30AM- 10:00AM |
Expert Talk on “The Importance of Flow Cytometry in Biomedical Research and Diagnostics” |
|
10.00 AM – 11.00 AM |
Understanding the technology of Spectral Flowcytometry and its difference from Conventional Flowcytometry |
|
11:00AM – 12:00PM |
Basics of Flowcytometry and cell sorting and its components (principlesandinstrumentation) |
|
12:00PM – 1:30PM |
Practical Demonstration of BigFoot Cell sorter:Components and Operation(including routine checks ofperformance)
|
|
1:30 PM – 2:30 PM |
Lunch |
|
Theme 2: Applications of Flowcytometer and cell sorter |
|
|
2:30 PM – 3:00 PM |
Application of Flowcytometer: Cell Cycle; Cell viability; Apoptosis; Immunophenotyping and Applications of Cell sorting |
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3:00 PM-3:30 PM |
Application in Detail: Cell Analysis using PI and Thermo Fisher Vibrant dye cycle |
|
3:30 PM-5:30 PM |
Wet Lab: Cell Cycle: Cell Preparation and Staining |
|
3:30 PM – 4:30 PM |
Batch 1: Cell Cycle sample run on BigFoot |
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4:30 PM – 5:30 PM |
Batch 2: Cell Cycle sample run on BigFoot |
|
5:30 PM – 6:00 PM |
Q & A session |
Day 2: Tuesday April 8, 2025
|
Time |
Program |
|
|
9:00AM- 10:00 AM |
Spectral Flowcytometry and Panel Design |
|
|
10:00 AM – 10:30 AM |
Understanding Basic Immunophenotyping: Immune Cell Population and their markers |
|
|
10:30 AM – 11:30 AM |
Application in Detail: Immunophenotyping- Surface and Intracellular: Choiceofrightfluorochromesandantibodies, Spectral overlap,Voltage settings, Importance ofexperiment, data acquisition andcontrols. |
|
|
11:30 AM – 1:30PM |
Wet Lab: Hands-on training in Peripheral Blood Mononuclear Cell Isolation and Discussion |
|
|
1:30 PM – 2:30 PM |
Lunch |
|
|
|
Batch 1 |
Batch 2 |
|
3:30 PM – 4:30 PM |
Wet Lab: Surface Antibody staining of cells |
Acquisition in BigFoot and Analysis |
|
Acquisition in BigFoot and Analysis Spectral overlap, |
Wet Lab: Surface Antibody staining of cells |
|
|
4:30 PM – 5:30 PM |
Data Analysis: Cell Cycle and Immunophenotyping |
|
|
5:30 PM – 6:30 PM |
Quiz and Valedictory |
|
| Patrons |
Prof. Shantikumar V Nair, |
Prof. Amit Kumar Dinda |
| Workshop Coordinator | Dr. Ashish Kumar Vyas | |
| Organizing Team | Dr. Anubhav Panday, Dr. Pankaj Jha, Dr. Sreeja Roy, Dr. Bhargab Kalita, Dr. Aparup Patra, Dr. Anuj Dilwaria and Mr. Vikas Jha | |
Target Audience
Mode of Selection : First cum first serve basis.
Note: The last date for registration is 31st March 2025.