Pre-requisites: Basic understanding of DNA chemistry and DNA biology
Total number of classes: 30
Syllabus
Isolation of chromosomal DNA from Escherichia coli;Agarose gel electrophoresis, Isolation of chromosomal DNA from human blood; Isolation of plasmid DNA from Escherichia coli; Nucleic acid quantification; Polymerase chain reaction (PCR), Restriction digestion, Restriction fragment length polymorphism (RFLP), RNA isolation from Escherichia coli; cDNA synthesis, Reverse Transcriptase PCR, DNA sequencing, Real time PCR.
Course Outcome
CO1 Explain the principles of the DNA & RNA isolation methods, PCR, agarose gel electrophoresis, sequencing methods.
CO2 Can isolate DNA, RNA, plasmids.
CO3 Can perform PCR, cDNA synthesis, RT-PCR, Real-time PCR, and sequencing.
CO4 Can follow general safety routines for laboratory work in molecular biology.
CO5 Can plan experimental work based on a protocol.
CO6 Can critically evaluate and discuss experimental results.
Programme Outcomes(PO)(As given by NBA and ABET)
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
|
c |
PO1 |
PO2 |
PO3 |
PO4 |
PO5 |
PO6 |
PO7 |
PO8 |
PO9 |
PO10 |
PO11 |
PO12 |
|
C O |
||||||||||||
|
CO 1 |
3 |
2 |
2 |
1 |
3 |
1 |
– |
1 |
3 |
– |
– |
1 |
|
CO 2 |
3 |
2 |
3 |
3 |
3 |
1 |
– |
1 |
3 |
– |
– |
1 |
|
CO 3 |
3 |
2 |
3 |
3 |
3 |
1 |
– |
1 |
3 |
– |
– |
1 |
|
CO 4 |
2 |
3 |
3 |
2 |
2 |
1 |
– |
– |
– |
– |
– |
1 |
|
CO 5 |
3 |
3 |
1 |
2 |
3 |
2 |
– |
– |
– |
– |
– |
1 |
Program Specific Outcomes. (PSO)
PSO 1 – Biochemical organization and cellular complexity in function
PSO 2 – Biomolecules in Medicine
PSO 3 – Molecular basis of disease
PSO 4 – Molecular technology in diagnosis and therapy
PSO 5 – Cellular based approaches in diagnosis and therapy
PSO 6 – Microorganisms in Medicine
PSO 7 – Nanoscale entities and its significance in Medicine
PSO 8 – Tissue architecture engineering in Medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and biological data use
|
– |
PSO1 |
PSO2 |
PSO3 |
PSO4 |
PSO5 |
PSO6 |
PSO7 |
PSO8 |
PSO9 |
PSO10 |
|
C O |
||||||||||
|
CO 1 |
2 |
– |
1 |
3 |
1 |
2 |
– |
– |
– |
1 |
|
CO 2 |
– |
– |
– |
3 |
1 |
1 |
– |
– |
– |
1 |
|
CO 3 |
– |
– |
– |
3 |
1 |
1 |
– |
– |
– |
1 |
|
CO 4 |
– |
– |
– |
3 |
– |
– |
– |
– |
– |
– |
|
CO 5 |
– |
– |
– |
3 |
– |
– |
– |
– |
– |
– |
Evaluation Pattern: 30+70 = 100
|
Internal Assessment – 30% |
||
|
Records |
Evaluation |
30% |
|
30% |
||
|
End Semester Examination- 50% |
||
|
Practical |
Exam |
60% |
|
Assessment |
Viva |
10% |
|
70% |
||
|
Total |
100% |
|