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Course Detail

Course Name Recombinant DNA Technology
Course Code 24MMD545
Program M. Sc. Molecular Medicine(MLM)
Semester 3
Credits 3
Campus Faridabad, Kochi

Syllabus

Unit 1

Restriction enzymes and cloning vectors: Host controlled restriction modification, Restriction endonucleases – types and classification, modifying enzymes used in molecular cloning – methylases, polymerases, ligases, kinases, phosphatases and nucleases,

Unit 2

Restriction enzymes and cloning vectors: Host controlled restriction modification,Restriction endonucleases – types and classification, modifying enzymes used in molecular cloning – methylases, polymerases, ligases, kinases, phosphatases and nucleases,

Unit 3

Cloning, Construction of Genomic and cDNA libraries: Introduction to cloning(conventional & recombination based: traditional cloning, TA cloning, Gateway cloning etc), vGene transfer techniques: Transformation, transfection and transduction, Strategies for construction of genomic libraries, Strategies for construction of cDNA libraries.

Unit 4

Selection and characterization of recombinant clones: Genetic Selection- insertional inactivation and alpha complementation, Labeling of nucleic acids, Immunological probes, Selection of recombinant clones-hybridization techniques, colony hybridization & library screening, hybrid arrest & hybrid release translation, DNA sequencing methods, DNA arrays.

Unit 5

Advanced techniques and applications: Genome editing- CRISPR-Cas system, TALENs &amp ZFNs, Site directed mutagenesis and RNA interference, Recombinant vaccines, Recombinant antibodies (Fab, scFv, sdAb), Disease diagnosis, Gene therapy- technologies, applications and regulations Next generation sequencing- principle, types and applications.

Objectives and Outcomes

Pre-requisites: Undergraduate level basic biology, biotechnology 

Total number of classes: 30

Syllabus

Course Outcome

CO1 Understand the fundamentals of molecular cloning

CO2 Understand the technical know-how on versatile techniques in recombinant DNA technology.

CO3 Gain knowledge of tools and strategies used in molecular cloning

CO4 Understand how to select and characterize recombinant clones

CO5 Understand basic and advanced molecular cloning concepts, applications and techniques


Programme Outcomes (PO) (As given by NBA and ABET)


PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

PO1

PO2

PO3

PO4

PO5

PO6

PO7

PO8

PO9

PO10

PO11

PO12

C O

CO 1

3

–

2

–

2

–

–

2

–

–

–

2

CO 2

3

1

2

2

3

–

–

–

–

–

–

1

CO 3

3

2

1

1

3

2

–

–

–

–

–

1

CO 4

3

2

3

3

–

1

–

–

–

–

–

1

CO 5

3

–

–

2

3

1

–

–

–

–

–

1

Program Specific Outcomes. (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine 

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine 

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

–

PSO1

PSO2

PSO3

PSO4

PSO5

PSO6

PSO7

PSO8

PSO9

PSO10

C O

CO 1

–

–

–

3

1

2

–

–

–

1

CO 2

–

–

–

3

–

_

–

–

–

1

CO 3

–

–

–

3

2

1

–

–

–

1

CO 4

–

–

–

3

–

–

–

–

–

–

CO 5

–

–

–

3

–

–

–

–

–

–


Evaluation Pattern: 50+50 = 100

Internal Assessment – 50% 

Periodical 1 

Exam 

20% 

Periodical 2 

Exam 

20% 

Continuous Assessment 

Assignment/Test/Quiz 

10% 

 

50%

End Semester Examination- 50% 

Theory Exam 

50% 

  

 

50%

Total

100%

Text Books / References

Text book

  • Principles of Gene Manipulation, Sandy B. Primrose, Richard Twyman, Bob Old, Wiley, 08-Feb-2002
  • From Genes to Genomes: Concepts and Applications of DNA Technology. Jeremy W Dale and Malcom von Schantz 2002 John Wiley & Sons, Ltd.

Reference

  • Gene Therapy Technologies, Applications and Regulations: From Laboratory to Clini. Anthony Meager 1999 John Wiley & Sons, Ltd.

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