Publication Type : Journal Article
Source : Letters in Applied Microbiology
Url : https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2006.01842.x
Campus : Amritapuri
School : School for Sustainable Futures
Year : 2006
Abstract :
Aim: To compare few phenotypic and genotypic characteristics of two desulfurizing bacterial strains, Mycobacterium phlei SM120-1 and Mycobacterium phlei GTIS10.
Methods and Results: In the present study, dibenzothiophene (DBT) desulfurizing activity, composition of fatty acids of cell membranes, DBT sulfone monoxygenase gene (bdsA) and the selection pressure applied during the growth and enrichment of the bacterial strains M. phlei SM120-1 and M. phlei GTIS10 were compared in our laboratory. The DBT desulfurization activity of M. phlei SM120-1 was found to be 0·17 ± 0·02 μmol 2-HBP min−1 (gram dry cell weight)−1 and that of the bacterial strain M. phlei GTIS10 was 1·09 ± 0·05 μmol 2-HBP min−1 (gram dry cell weight)−1. Fatty acid methyl ester analysis of cell membranes of these two bacterial strains in the presence of light gas oil showed that both the strains had different fatty acid profiles in their cell membranes. Comparison of the full gene sequences of the desulfurization gene bdsA in the two bacterial strains showed significant difference in the bdsA gene sequences. There was a significant difference observed in the selection pressure applied during the growth and enrichment of the two bacterial strains.
Conclusions: The results of the comparative study of the bacterial strains, M. phlei SM120-1 and M. phlei GTIS10 showed that there were considerable differences in the phenotypic and genotypic characteristics of these two strains.
Significance and Impact of Study: The present study would broaden the understanding of biodesulfurization trait at intra-species level.
Cite this Research Publication : K. Srinivasaraghavan, P.M. Sarma, B. Lal,”Comparative analysis of phenotypic and genotypic characteristics of two desulfurizing bacterial strains, Mycobacterium phlei SM120-1 and Mycobacterium phlei GTIS10”, Letters in Applied Microbiology 2006.