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Publication Type : Journal Article
Thematic Areas : Biotech
Publisher : Acta Horticulturae
Source : Acta Horticulturae,(Proc. WOCMAP II 1999) Volume, vol. 3, pp. 281–288, 1999
Campus : Amritapuri
School : School of Biotechnology
Center : Cancer Biology
Department : biotechnology
Year : 1999
Abstract : A rapid mass propagation method for Santalum album through in vitro cultivation in liquid media and airlift bioreactor has been developed. Embryogenesis, maturation, and germination were performed in the same bioreactor by the removal of exhausted medium and the input of fresh medium without any subculturing. In comparison to the propagation in agar solidified medium, this process of propagation increases the efficiency of production reduces the time required, and minimizes abnormalities. In the bioreactor, about 3000 somatic seedlings were obtained (59.3% were abnormal) in 6 weeks time, per liter of medium, against about 800 (88.9% abnormality) in 12 weeks in the case of solid medium. About 70% of the somatic seedlings grew to healthy plantlets and tolerated hardening trials, something that was rarely possible for somatic seedlings raised on solid media.
Cite this Research Publication : S. Das, Das, S., Dr. Sanjay Pal, Mujib, A., Sahoo, S. S., Ponde, N. R., S Gupta, D., and Dey, S., “A novel process for rapid mass propagation of the aromatic plant Santalum album in liquid media and bioreactor”, Acta Horticulturae,(Proc. WOCMAP II 1999) Volume, vol. 3, pp. 281–288, 1999