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Course Detail

Course Name Biochemistry Lab
Course Code 22MMD584
Program M. Sc. Molecular Medicine(MLM)
Semester 2
Credits 2
Campuses Faridabad, Kochi

Syllabus

Syllabus

Experiment 1: Preparation of Laboratory Reagents with different concentrations, PH measurement.

Experiment 2: Protein estimation: Lowry method and spectrometry principles.

Experiment 3: Protein estimation: Biuret method / Bicinchoninic acid

Experiment 4: Enzyme Estimation (Serum Amylase) – Iodometric Method

Experiment 5: Amino acid Estimation by Biuret Test, Millon’s Test, Hopkin’s Cole Test, Xanthoproteic Test, Lead Acetate Test

Experiment 6: Protein Separation by SDS-PAGE and Western Blotting.

Experiment 7: Chromatographic techniques (Eg., Thin Layer chromatography or high performance liquid chromatography (HPLC)

Summary

Pre-requisites: Basic understanding of biology and chemistry

Total number of classes: 30

Course Outcome

Course Outcome

CO1:   Understand the basics of the measure of solution concentration

CO2:   Understand the importance of protein estimation, different types of protein estimation (Eg., Lowry method, Biuret method etc)

CO3:   Understand how to quantify enzyme level in serum (amylase enzyme)

CO4:   Determine the presence of amino acids in a given sample and know the advantages / disadvantages of different method of detection.

CO5:   Evaluate separation of proteins in the sample by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and chromatographic techniques

Program Outcome

PO1: Bioscience Knowledge

PO2:  Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO1 3 3 2 1 1 2 3 2 2
CO2 3 3 2 1 1 2 3 2 2
CO3 3 3 2 1 1 2 3 2 2
CO4 3 3 2 1 3 2 3 2 2
CO5 3 3 2 1 2 2 3 2 2

Program Specific Outcomes (PSO)

PSO 1 – Biochemical organization and cellular complexity in function

PSO 2 – Biomolecules in Medicine

PSO 3 – Molecular basis of disease

PSO 4 – Molecular technology in diagnosis and therapy

PSO 5 – Cellular based approaches in diagnosis and therapy

PSO 6 – Microorganisms in Medicine

PSO 7 – Nanoscale entities and its significance in Medicine

PSO 8 – Tissue architecture engineering in Medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and biological data use

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10
CO1 2
CO2 1 2 2 2
CO3 1 2 2 2
CO4 1 2 2 2
CO5 1 2 2 2

Evaluation Pattern: 30+70 = 100

Internal Assessment – 30% 
Records Evaluation 30%
30%
End Semester Examination- 50% 
Practical Exam 60%
Assessment Viva 10%
70%
Total 100%

Textbooks/ Reference Books

Textbooks:

  1. T. Plummer. An Introduction to Practical Biochemistry, McGraw Hill Education; 3rd edition, 2017.
  2. Rodney Boyer. Biochemistry laboratory: Modern Theory and Techniques (2nd Edition), Pearson, 2011.

References:

  1. Chaykin Biochemistry Laboratory Techniques, 169pages. John Wiley & Sons Inc., blew York.

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