Publication

Abstract : The Supplementary Tables 1-3 report functional cellular parameters at P40-P50 in C57BL/6J and PrP0/0 mouse granule cells. No statistically significant differences between the two strains were found in any of the parameters. These parameters were also compared to those measured at P17-P22 and reported in the corresponding tables of the main text. Compared to juvenile, adult granule cells in both C57BL/6J and PrP0/0 showed a lower input resistance (Rm in Table 1 vs. Table Supplementary 1: p< 0.01, unpaired t-test) associated with a slower membrane time constant (τm= RmCm in Table 1 vs. Table Supplementary 1: p< 0.01, unpaired t-test) and a smaller EPSP peak amplitude (EPSPampl in Table 2 vs. Table Supplementary 2: p< 0.01, unpaired t-test). Moreover, the spikes were significantly faster (APHW in Table 2 vs. Table Supplementary 2: p< 0.001, unpaired t-test) and the peak Na+ and K+ currents somehow (although not significantly) smaller, maybe reflecting displacement of channels in the axon (Goldfarb et al., 2007) or subtle changes in gating kinetics. None of the other excitable parameters did significantly differ at the two ages. Interestingly, the comparison reveals that LTP at P40-P50 has very similar changes as at P17-P22, indicating the persistence of mossy fiber–granule cells long-term synaptic plasticity in adulthood.