Back close
Admissions

Course Detail

Course Name Analytical Biochemistry
Program BSc. in Biotechnology, BSc. in Microbiology
Credits Three
Year Taught 2019

Syllabus

  • Methods of protein extraction, Protein quantitation: Biuret, Lowry, BCA and Bradford methods, Protein precipitation: Salting-in, Salting-out, Effect of organic solvents and polymers. Protein separation: Dialysis, Ultrafiltration, Centrifugation.
  • Chromatography: Partition coefficient, Retention, Resolution, Capacity factor, theoretical plate, van Deemter curve, Gel flitration chromatography, Ion exchange chromatography, Affinity chromatography, Hydrophobic interaction chromatography, Hydroxyapatite chromatography, Paper chromatography, Thin layer chromatography, Reversed-phase chromatography, Fundamentals of high performance chromatography.
  • Electrophoresis: Native PAGE, SDS-PAGE, Isoelectric focussing, 2D-PAGE, Capillary electrophoresis.
  • Spectroscopy: Fundamentals of UV Spectroscopy, Spectrophotometer, Fundamentals of fluorescence spectroscopy, Spectrofluorometer.
  • Mass Spectrometry: Electron Impact, Chemical Ionization and Fast atom Bombardment mass spectrometers.
Syllabus - Year Taught
2015 2014

Text Books

Resources

  1. Biochemistry: Voet and Voet
  2. Protein Purification Techniques: Simon Roe
  3. Protein Purification: Robert K. Scopes
  4. Physical Biochemistry: David Sheehan
  5. Practical Biochemistry: Keith Wilson and John Walker
  6. Mass Spectrometry for Biotechnology: Gary Siuzdak

 

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now