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Resazurin based redox dye as an indicator for monitoring wastewater biological activity

Publication Type : Conference Paper

Thematic Areas : Biotech

Publisher : Pollut. Res.

Source : Innovative Strategies for Sustainable Water Management (ISSWM-2017), Punjab, India (2018)

Url : http://www.envirobiotechjournals.com/article_abstract.php?aid=8678&iid=249&jid=4

Campus : Amritapuri

School : School of Biotechnology

Center : Biotechnology, Sanitation Biotechnology

Department : biotechnology

Verified : Yes

Year : 2018

Abstract : Resazurin (alamarBlue®) based assay was considered as a quick tool to monitor biological activity and was preferred over Biochemical Oxygen Demand (BOD) assay which generally takes 3-5 days of incubation. It was designed to quantitatively measure cell viability and was based on the biological reduction of fluorometric redox indicator resazurin (purple) to resorufin (pink) which was generally performed for aerobic organisms. In our study on wastewater biological activity, we observed that resazurin can be used as an indicator of anaerobic respiration as well. Thus, resazurin based fluorometric assays can be widely used as an effective tool for the monitoring the sewage wastewaters.

Cite this Research Publication : Pradeesh Babu, S. J. Poornendu, Amrita Salim, Ajith Madhavan, Dr. Bipin G. Nair, and Dr. Sanjay Pal, “Resazurin based redox dye as an indicator for monitoring wastewater biological activity”, in Innovative Strategies for Sustainable Water Management (ISSWM-2017), Punjab, India, 2018.

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