Publication Type : Journal Article
Thematic Areas : Nanosciences and Molecular Medicine, Waste Management & Infrastructure
Publisher : Eur J Pharm Sci,
Source : Eur J Pharm Sci, Volume 96, p.193-206 (2017)
Url : http://www.sciencedirect.com/science/article/pii/S0928098716303591
Keywords : In the present study chitin nanogel loaded with anti-psoriatic drug clobetasol was developed (CLCNG) for its topical delivery in psoriasis. CLCNG had the particle size of 132±14nm, with gel like consistency, stability in refrigerator, having higher drug release properties at acidic pH. CLCNG exhibited significant toxicity towards HaCaT and THP-1cell lines by MTT assay. The uptake of nanogel by HaCaT cell lines was confirmed by fluorescent microscopy. CLCNG at 0.35mg/ml exhibited significant anti-inflammatory activity with an average of 65% and 70% inhibition in COX and LOX activities expressed in THP-1 cells. In vitro skin permeation studies revealed the increased transdermal flux with fragmented stratum corneum and loosened epidermal layers in CLCNG treated samples, compared with control drug solution. The in vivo anti-psoriatic studies done on imiquimod model confirmed the potential benefits of the nanogel for the topical delivery of clobetasol in psoriasis.
Campus : Kochi
School : College of Nursing
Center : Amrita Center for Nanosciences and Molecular Medicine Move
Department : Obstetrics and Gynecological Nursing
Abstract : In the present study chitin nanogel loaded with anti-psoriatic drug clobetasol was developed (CLCNG) for its topical delivery in psoriasis. CLCNG had the particle size of 132±14nm, with gel like consistency, stability in refrigerator, having higher drug release properties at acidic pH. CLCNG exhibited significant toxicity towards HaCaT and THP-1cell lines by MTT assay. The uptake of nanogel by HaCaT cell lines was confirmed by fluorescent microscopy. CLCNG at 0.35mg/ml exhibited significant anti-inflammatory activity with an average of 65% and 70% inhibition in COX and LOX activities expressed in THP-1 cells. In vitro skin permeation studies revealed the increased transdermal flux with fragmented stratum corneum and loosened epidermal layers in CLCNG treated samples, compared with control drug solution. The in vivo anti-psoriatic studies done on imiquimod model confirmed the potential benefits of the nanogel for the topical delivery of clobetasol in psoriasis.
Cite this Research Publication : R. Panonnummal, Jayakumar, R., and Sabitha, M., “Comparative anti-psoriatic efficacy studies of clobetasol loaded chitin nanogel and marketed cream.”, Eur J Pharm Sci, vol. 96, pp. 193-206, 2017.
