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Alpha 1, 5- L endo-arabinanase production media formulation and optimization using cost effective substrate

Publication Type : Journal Article

Publisher : Volume 9, p.40-45

Source : Volume 9, p.40-45 (2016)

Url : https://www.researchgate.net/publication/301554779_Alpha_1_5-_L_endo-arabinanase_production_media_formulation_and_optimization_using_cost_effective_substrate

Campus : Amritapuri

School : School of Biotechnology

Year : 2016

Abstract : A new, cost effective media was formulated with soy chunk powder (SCP) as the nutrient source. Growth parameters such as temperature, pH and substrate concentration were optimized. Temperature of 300C, pH of 4 and 1.5% (w/v) of substrate concentration, constitute the optimal condition for the maximal production of the enzyme. The enzyme production peaked in the 5th day of incubation with a maximal enzyme activity of 1.218 U/mg which is 42% as efficient as production in media employing pure substrate. Aspergillus niger isolated from the soil samples collected from the precincts of the campus was employed in the current study.

Cite this Research Publication : Sindhu Shetty K., Madhavan, A., Ben, C. S. Sreejith, Lekshmi, R., Kumar, B. S., Govind, K., Aswathy, S., and Shanmugham, S., “Alpha 1, 5- L endo-arabinanase production media formulation and optimization using cost effective substrate”, vol. 9, pp. 40-45, 2016.

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